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Cheek Brush Sampling & Shipping for the MDR1 Test
It is IMPORTANT THAT FOOD PARTICLES ARE NOT INCLUDED with the cheek
and gum cells sampled from the mouth. Sample dogs when no food
particles are present in the mouth. In the morning before eating is
ideal. Otherwise, wait for at least 30 min. after eating for the food
particles to clear out of the mouth.
Label the brushes
Use the two brushes provided for each dog sampled.
Label the paper
backing of each brush package with the dog’s name, and your first and
last name (or name of the Veterinary Hospital), using a pen or
permanent marker.
Take the samples
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Remove the brush from the package.
Use the two brushes provided for each dog sampled.
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Hold
the brush handle between your thumb and forefinger.
Roll the inner surface of the upper lip to the outside by
inserting a finger in the corner of the mouth and pulling the lip
upwards.
Place the bristles of the brush on the inner surface of the lip. |
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Release the lip, leaving the brush inside the mouth and steady the
dog's muzzle by circling the nose with one hand, gently keeping
the dog's mouth closed.
Move the brush back and forth for a short distance in the space
between the lip and gum, twirling the brush around with your thumb
and forefinger as it is moved back and forth. Do not push the tip
of the brush into the soft tissues of the mouth. The side bristles
will be brushing on the surface of the gums and inner side of the
lip.
Continue for 20-30
seconds (about 20 to 30 back and forth motions) using the same pressure you would use to brush your teeth. |
Repeat sampling on the other side of the mouth using the remaining
brush. If multiple dogs are being sampled wash your hands between dog
samplings.
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After
collecting the sample, the brush should look different than the
unused brush. The bristles may be darker in dogs with pigmented
gums or you may see a light film on the brush bristles.
An unused
brush is shown on the left, and a used brush on the right.
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Tips
A few swipes will not get enough cheek cells to run the test! Be
sure to brush long enough.
Follow this link and
complete the information about yourself and include with the
samples
* PCR
for this service is performed pursuant to an agreement with Roche
Molecular Systems, Inc.
US and foreign patents pending for MDR1 testing.
VCPL@vetmed.wsu.edu
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