College of Veterinary Medicine

Veterinary Clinical Pharmacology Lab

Instructions for Use of the Test Kit & Submission of Samples*


Cheek Brush Sampling & Shipping for the MDR1 Test

It is IMPORTANT THAT FOOD PARTICLES ARE NOT INCLUDED with the cheek and gum cells sampled from the mouth. Sample dogs when no food particles are present in the mouth. In the morning before eating is ideal. Otherwise, wait for at least 30 min. after eating for the food particles to clear out of the mouth.

Label the brushes
Use the two brushes provided for each dog sampled.

Label the paper backing of each brush package with the dog’s name, and your first and last name (or name of the Veterinary Hospital), using a pen or permanent marker.

Take the samples
 

    Remove the brush from the package.

    Use the two brushes provided for each dog sampled.

Hold the brush handle between your thumb and forefinger.

Roll the inner surface of the upper lip to the outside by inserting a finger in the corner of the mouth and pulling the lip upwards.

Place the bristles of the brush on the inner surface of the lip.

Release the lip, leaving the brush inside the mouth and steady the dog's muzzle by circling the nose with one hand, gently keeping the dog's mouth closed. 

Move the brush back and forth for a short distance in the space between the lip and gum, twirling the brush around with your thumb and forefinger as it is moved back and forth. Do not push the tip of the brush into the soft tissues of the mouth. The side bristles will be brushing on the surface of the gums and inner side of the lip.

Continue for 20-30 seconds (about 20 to 30 back and forth motions) using the same pressure you would use to brush your teeth.

Repeat sampling on the other side of the mouth using the remaining brush. If multiple dogs are being sampled wash your hands between dog samplings.
 

After collecting the sample, the brush should look different than the unused brush. The bristles may be darker in dogs with pigmented gums or you may see a light film on the brush bristles.

 An unused brush is shown on the left, and a used brush on the right.

 


Tips
A few swipes will not get enough cheek cells to run the test!  Be sure to brush long enough.

Follow this link and complete the information about yourself and include with the samples


* PCR for this service is performed pursuant to an agreement with Roche Molecular Systems, Inc.
US and foreign patents pending for MDR1 testing.

Last Edited: Sep 04, 2007 1:21 PM
Veterinary Clinical Pharmacology Laboratory, PO Box 609 , Washington State University, Pullman WA 99163-0609, 509-335-3745, Contact Us Safety Links