Washington Animal Disease Diagnostic Lab

• User Guide
• Fee Schedule
• Diagnostic Updates

  

Purpose: Immunohistochemistry involves the detection of specific chemical substances (infectious agents or cellular markers) within tissue by the use of derived antibodies to the substances. Antibodies (polyclonal or monoclonal) are applied to tissue sections and allowed to bind to their corresponding antigen. A detection system is then employed to identify the location of these antibodies using amplified marker molecules that can be visually recorded. These identifiable substrates offer a documentation of localization that can be seen through the microscope at the cellular level. In this way, individual cells or groups of cells can be monitored for the presence of the specific antigen.

The techniques for detection are numerous, but the overall technology is essentially the same. WADDL currently utilizes the streptavidin-biotin complex immunoperoxidase detection methods for mammalian tissues and peroxidase anti-peroxidase methods for all avian tissues. The results are interpreted and reported by the pathologist.

Samples:Formalin-fixed tissues or formalin-fixed paraffin-embedded tissues

Controls

Positive and negative control slides provided with each ordered test:

1. Positive control: Positive tissue incubated with positive antibody
2. Negative control: Test tissue incubated with matched concentration of irrelevant antibody

Ordering Information: Include animal species and tissue type for each case

Immunostain: Chromogen for all IHC assays is amino-ethyl-carbazole (AEC), which is red. Counterstain for all IHC assays is Mayer's hematoxylin, which is blue.

Test	Fees	Specimen	Shipping Container	Max. Turnaround	Comments
Infectious Agents	Fee schedule	Tissues in 10% Formalin OR Formalin fixed paraffin-embedded tissue.	Leakproof jar or Styrofoam mailer	7 working days	Results interpreted as “Detected” or “Not Detected” and reported separate from pathology report.
Tumor Markers	Fee schedule	Tissues in 10% Formalin OR Formalin fixed paraffin-embedded tissue.	Leakproof jar or Styrofoam mailer	7 working days	Results interpreted as “positive” or “negative” and reported as part of pathology report

Infectious Agents	Clinical Disease	Comments
Bovine Coronavirus	Enteric
Bovine Herpes Virus-1 (IBR)	Abortion, Respiratory
Bovine Respiratory Syncytial Virus	Respiratory
Bovine Viral Diarrhea Virus	Abortion, Enteric, Respiratory, Systemic
Bovine Viral Diarrhea - Ear Notch	BVD Persistent infection (PI)	BVD Antigen ELISA preferred
Canine Adenovirus	Canine Infectious Hepatitis
Canine Parvovirus	Enteric
Chlamydiaceae	Systemic
Coxiella burnetii	Abortion, Enteric, Respiratory, Systemic
Equine Herpes Virus-1	Abortion, Respiratory
Feline Coronavirus	Feline Infectious Peritonitis
Feline Parvovirus	Enteric
Leptospira spp.	Abortion, Systemic
Listeria monocytogenes	Abortion, Neurologic
Parainfluenza-3	Respiratory
Porcine Coronavirus (TGE)	Enteric
Prion-CWD	Neurologic	Reportable disease
Prion-Scrapie	Neurologic	Reportable disease
Sarcocystis neurona	Neurologic
Tumor Markers	Neoplasm	Cell Specificity
CD3	Lymphosarcoma	T-lymphocyte
CD79a	Lymphosarcoma	B-lymphocyte
Chromatogranin A	Neuroendocrine	Neuroendocrine
Cytokeratin (Pan)	Carcinoma	Epithelial cell
Desmin	Rhabdomyosarcoma	Skeletal muscle
Factor VIII	Hemangiosarcoma	Endothelial cell
Glial Fibrillary Acidic Protein (GFAP)	CNS (astrocytoma, ependymoma)	Astrocyte, ependymal cell
Lambda light chain	Plasmacytoma	Plasma cell
Lysozyme	Histiocytosis	Macrophage, neutrophil
Mac 387	Histiocytosis	Macrophage
Melan A	Melanoma	Melanocyte
Muscle Actin	Leiomyosarcoma, Rhabdomyosarcoma	Skeletal muscle myocyte, Smooth muscle myocyte
Neurofilament	Ganglioneuroma	Neuron
Synaptophysin	Neuroendocrine	Neuroendocrine
Vimentin	Sarcoma	Mesenchymal cell