Immunohistochemistry (IHC)
Purpose: Immunohistochemistry involves the detection of
specific chemical substances (infectious agents or cellular
markers) within tissue by the use of derived antibodies to the
substances. Antibodies (polyclonal or monoclonal) are applied to
tissue sections and allowed to bind to their corresponding
antigen. A detection system is then employed to identify the
location of these antibodies using amplified marker molecules
that can be visually recorded. These identifiable substrates
offer a documentation of localization that can be seen through
the microscope at the cellular level. In this way, individual
cells or groups of cells can be monitored for the presence of
the specific antigen.
The techniques for detection are numerous, but the overall
technology is essentially the same. WADDL currently utilizes
multiple detection systems including the streptavidin-biotin
complex immunoperoxidase detection method, an alkaline
phosphatase Fast Red/Luxol detection method, and peroxidase
anti-peroxidase method depending on the specific test. The
results are interpreted and reported by the pathologist.
Samples:Formalin-fixed tissues or formalin-fixed
paraffin-embedded tissues
Controls
Positive and negative control slides provided with each
ordered test:
- Positive control: Positive tissue incubated with
positive antibody
- Negative control: Test tissue incubated with matched
concentration of irrelevant antibody
Ordering Information: Include animal species and
tissue type for each case
Immunostain:
Chromogen for all IHC
assays
depends upon the test
run and include both amino-ethyl-carbazole (AEC), which is
red/brown and UltraView Red, which is bright magenta..
Counterstain for all IHC assays is Mayer's hematoxylin, which is
blue.
|
Test |
Fees |
Specimen |
Shipping Container |
Max. Turnaround |
Comments |
|
Infectious Agents
|
Fee schedule
|
Tissues in 10% Formalin OR Formalin fixed paraffin-embedded
tissue. |
Leakproof jar or Styrofoam mailer |
7 working days
|
Results interpreted as “Detected” or “Not Detected” and
reported separately from pathology report. |
|
Tumor Markers |
Fee schedule
|
Tissues in 10% Formalin OR Formalin fixed paraffin-embedded
tissue. |
Leakproof jar or Styrofoam mailer |
7 working days
|
Results interpreted as “positive” or “negative” and reported
as part of pathology report |
|
Infectious
Agents |
Clinical Disease |
Comments |
|
Bovine Herpes Virus-1 (IBR) |
Abortion, Respiratory |
|
|
Bovine Respiratory Syncytial Virus |
Respiratory |
|
|
Bovine Viral Diarrhea Virus |
Abortion, Enteric, Respiratory, Systemic |
|
|
Bovine Viral Diarrhea - Ear Notch |
BVD Persistent infection (PI) |
BVD Antigen ELISA preferred |
|
Canine Parvovirus |
Enteric |
|
|
Equine Herpes Virus-1 |
Abortion, Respiratory |
|
|
Feline Coronavirus |
Feline Infectious Peritonitis |
|
|
Feline Parvovirus |
Enteric |
|
|
Leptospira spp. |
Abortion, Systemic |
|
|
Listeria monocytogenes |
Abortion, Neurologic |
|
|
Porcine Coronavirus (TGE) |
Enteric |
|
|
Prion-CWD |
Neurologic |
Reportable disease |
|
Prion-Scrapie |
Neurologic |
Reportable disease |
|
Tumor Markers |
Neoplasm |
Cell Specificity |
|
CD3 |
Lymphosarcoma |
T-lymphocyte |
|
CD20 |
Lymphosarcoma |
B-lymphocyte |
|
Cytokeratin (Pan) |
Carcinoma |
Epithelial cell |
|
Desmin |
Rhabdomyosarcoma |
Skeletal muscle |
|
Factor VIII |
Hemangiosarcoma |
Endothelial cell |
|
Lambda light chain |
Plasmacytoma |
Plasma cell |
|
Melan A |
Melanoma |
Melanocyte |
|
Muscle Actin |
Leiomyosarcoma, Rhabdomyosarcoma |
Skeletal muscle myocyte, Smooth muscle myocyte |
|
Neurofilament |
Ganglioneuroma |
Neuron |
|
Vimentin |
Sarcoma |
Mesenchymal cell |
