General Policies
Bacteriology
Virology
Molecular Diagnostics
 

Pathology
Parasitology
Toxicology

General Policies

1. All cases except those exclusively for toxicology will be assessed an accession fee of $10.00. An accession is defined as specimen(s) of one animal species or miscellaneous substances belonging to one owner and submitted at one time.
    
2. Clinical pathology services (e.g., hematology and cytology) are provided by the Department of Veterinary Clinical Sciences (VCS) (509-335-0745). These services are billed by VCS.
    
3. A FAX, mail or electronic report is provided at no charge.
    
4. Samples sent for analysis to outside labs are billed for any additional direct costs incurred. Handling fee (includes postage) is $15.00.
    
5. Fees are subject to change without notice.
    
6. Use Federal Express Account #2112-4551-4 for discounted shipping. Freight costs will be invoiced with case charges. FOR WADDL SUBMISSIONS ONLY.

Shipping address

155N Bustad Hall
Pullman, WA 99164-7034

Aquatic Health Inspection Testing

Aquatic animal health testing is performed in accordance with the guidelines outlined in both the OIE Manual of Diagnostic Tests for Aquatic Animals and the AFS-USFWS Blue Book.

Turn around time for all health inspections is 29 days based on the requirements set forth in both manuals for virology testing.

Two to four weeks advanced notice for scheduling purposes is stongly recommended on all health inspection samplings

Test	Fees		Specimen Type	Comments
Culture Identification of Aeromonas salmonicida and Yersinia ruckeri (per fish)	1.50	2.70	Kidney streaks on TSA, BHI or Blood Agar plates	Maintain samples below 15ºC
Aerobic culture, contaminated plate surcharge (per tissue)	3.00	5.40
Renibacterium salmoninarum (BKD)      BKD FAT (per test)      BKD ELISA (per test)	2.40 4.50	3.60 8.10	Fresh Kidney or Ovarian Fluid	Maintain samples below 15ºC
Piscirickettsia salmonis Direct tissue touch exam	3.00	4.50	Fresh Kidney	Maintain samples below 15ºC
M. cerebralis digest, small to medium sized fish (per test)	8.00	14.40	Head, skull section, gill arches	Maintain samples below 15ºC
M. cerebralis digest, large fish (per test)	10.00	18.00	Intestinal Mucosa	Maintain samples below 15ºC
Ceratomyxa shasta by intestinal smear (per test)	3.00	5.00
Bothriocephalus acheilognathi (Asian tapeworm) (per test)	3.00	5.00	Anterior 1/3 of intestine	Maintain samples below 15ºC
Viral Culture (per test per cell line) Viral pathogens routinely tested for include: Epizootic hematopoietic necrosis virus Infectious hematopoietic necrosis virus Infectious pancreatic necrosis virus Oncorhynchus masou virus Viral hemorrhagic septicemia virus Infectious salmon anemia virus Spring Viremia of Carp (Contact lab regarding additional pathogens)	10.50	17.00
Aquatic Diagnostic Testing
Test	Fees		Specimen Type	Comments
Bacteriology	in state	out of state
Aerobic culture (per tissue)	7.00	10.50	Fresh kidney, spleen, liver, brain; bacterial swab; streaked TSA, BHI or Blood Agar plates	Maintain samples below 15ºC
Anerobic culture (per tissue)	6.50	9.75	Fresh kidney, spleen, liver, brain; bacterial swab; streaked TSA, BHI or Blood Agar plates	Maintain samples below 15ºC
Antimicrobial susceptibility	12.00	15.00
Renibacterium salmoninarum (BKD) culture (per test)	8.50	15.30	Fresh Kidney	Maintain samples below 15ºC
Piscirickettsia salmonis culture (per test)	20.00	30.00	Fresh Kidney	Maintain samples below 15ºC
Fungal culture (genus)*	11.00	15.75
Mycobacterium (genus)*	15.75	22.50
*For speciation see molecular diagnostics
Virology	in state	out of state
Viral isolation in cell culture (up to 4 cell lines)	70.00	105.00	Fresh kidney/spleen/pyloric caeca/gill filliment	Maintain samples below 15ºC
Each additional cell line
Cell lines in active culture include: Epithelioma Papulosum Cyprini (EPC) Chinook Salmon Embryo (CHSE-214) Salmon Head Kidney (SHK-1) Fat Head Minnow (FHM) Bluegill Fry (BF-2) For questions regarding cell lines not listed, please contact WADDL
Parasitology	in state	out of state
External parasite screen on fresh tissues (per fish)	2.40	3.60	live fish
Pathology	in state	out of state
Necropsy (includes gill wet prep/mucus-skin scrape)      Fish 1 -5      Each additional fish	25.00   5.00	37.50 7.50	live fish
Histopathology-Tissues from up to 5 fish      Each additional fish	25.00 10.00	37.50 15.00	Formalin fixed whole fish, tissues
External parasite screen by Histopathology (per slide)	5.00	9.00	Formalin fixed whole fish, tissues
Myxobolus cerebralis histopathology (per slide)	5.00	9.00	Formalin fixed head, skull section, gills
Sample collection at WADDL	in state	out of state	Whole fish
Mini sampling (per fish)	2.00	3.00
Standard sampling (per fish)	3.60	5.40
Sampling kit (plus freight)	15.00	27.00
Molecular Diagnostics	in state	out of state
Organism identification by PCR			tissue, culture media
Yersinia ruckeri	20.00	30.00
Flavobacterium psychrophilum	35.00	52.50
Renibacterium salmoninarum (BKD)	55.00	82.50
Mycobacterium spp./Fungal speciation
Direct PCR/sequencing	80.00	120.00
Cloning/sequencing	150.00	225.00
Toxicology	in state	out of state
Water quality analysis (per test) (ammonia, nitrite, hardness, pH, alkalinity, chlorine)	15.00	22.50	water
For the evaluation of additional chemicals or substances please contact WADDL

(509) 335-9696, FAX (509) 335-7424

e-mail: waddl@vetmed.wsu.edu

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