Aquatic animal health testing is performed in accordance with
the guidelines outlined in both the OIE Manual of Diagnostic
Tests for Aquatic Animals and the AFS-USFWS Blue Book.
Turn around time for all health inspections is 29 days based
on the requirements set forth in both manuals for virology
testing.
Two to four weeks advanced notice for scheduling purposes is
stongly recommended on all health inspection samplings
|
Test |
Fees
 |
Specimen Type |
Comments |
|
Culture Identification of
Aeromonas salmonicida and Yersinia ruckeri
(per fish) |
1.50
|
2.70 |
Kidney streaks on TSA, BHI or Blood Agar plates |
Maintain samples below 15ºC |
|
Aerobic culture, contaminated plate surcharge (per
tissue)
|
3.00
|
5.40 |
|
|
Renibacterium salmoninarum (BKD)
BKD FAT (per test)
BKD ELISA (per test) |
2.40
4.50 |
3.60
8.10 |
Fresh Kidney or Ovarian Fluid |
Maintain samples below 15ºC |
|
Piscirickettsia salmonis Direct tissue touch exam
|
3.00
|
4.50 |
Fresh Kidney
|
Maintain samples below 15ºC |
|
M. cerebralis digest, small to medium sized fish
(per test) |
8.00 |
14.40 |
Head, skull section, gill arches |
Maintain samples below 15ºC |
|
M. cerebralis digest, large fish (per test) |
10.00 |
18.00 |
Intestinal Mucosa |
Maintain samples below 15ºC |
|
Ceratomyxa shasta by intestinal smear (per test) |
3.00 |
5.00 |
|
|
|
Bothriocephalus acheilognathi (Asian tapeworm)
(per test) |
3.00 |
5.00 |
Anterior 1/3 of intestine |
Maintain samples below 15ºC |
|
Viral Culture (per test per cell line)
Viral pathogens routinely tested for include:
Epizootic hematopoietic necrosis virus
Infectious hematopoietic necrosis virus
Infectious pancreatic necrosis virus
Oncorhynchus masou virus
Viral hemorrhagic septicemia virus
Infectious salmon anemia virus
Spring Viremia of Carp
(Contact lab regarding additional pathogens) |
10.50 |
17.00 |
|
|
Aquatic Diagnostic Testing
|
|
Test |
Fees |
Specimen Type |
Comments |
|
Bacteriology |
in state |
out of state |
|
|
|
Aerobic culture (per tissue)
|
7.00
|
10.50 |
Fresh kidney, spleen, liver, brain; bacterial swab;
streaked TSA, BHI or Blood Agar plates |
Maintain samples below 15ºC |
|
Anerobic culture (per tissue)
|
6.50
|
9.75 |
Fresh kidney, spleen, liver, brain; bacterial swab;
streaked TSA, BHI or Blood Agar plates |
Maintain samples below 15ºC |
|
Antimicrobial susceptibility
|
12.00
|
15.00 |
|
|
|
Renibacterium salmoninarum (BKD) culture (per
test) |
8.50
|
15.30 |
Fresh Kidney |
Maintain samples below 15ºC |
|
Piscirickettsia salmonis culture (per test) |
20.00
|
30.00 |
Fresh Kidney |
Maintain samples below 15ºC |
|
Fungal culture (genus)*
|
11.00
|
15.75 |
|
|
|
Mycobacterium (genus)*
|
15.75
|
22.50 |
|
|
|
*For speciation see
molecular diagnostics |
|
Virology |
in state |
out of state |
|
|
|
Viral isolation in cell culture (up to 4 cell lines) |
70.00
|
105.00 |
Fresh kidney/spleen/pyloric caeca/gill filliment |
Maintain samples below 15ºC |
|
Each additional cell line |
|
|
|
|
Cell lines in active culture include:
Epithelioma Papulosum Cyprini (EPC)
Chinook Salmon Embryo (CHSE-214)
Salmon Head Kidney (SHK-1)
Fat Head Minnow (FHM)
Bluegill Fry (BF-2)
For questions regarding cell lines not listed, please
contact WADDL |
|
Parasitology |
in state |
out of state |
|
|
|
External parasite screen on fresh tissues (per fish) |
2.40
|
3.60 |
live fish |
|
|
Pathology |
in state |
out of state |
|
|
Necropsy (includes gill wet prep/mucus-skin scrape)
Fish 1 -5
Each additional fish |
25.00
5.00 |
37.50
7.50 |
live fish |
|
Histopathology-Tissues from up to 5 fish
Each additional fish |
25.00
10.00 |
37.50
15.00 |
Formalin fixed whole fish, tissues |
|
|
External parasite screen by Histopathology (per slide) |
5.00 |
9.00 |
Formalin fixed whole fish, tissues |
|
|
Myxobolus cerebralis histopathology (per slide) |
5.00 |
9.00 |
Formalin fixed head, skull section, gills |
|
|
Sample collection at WADDL |
in state |
out of state |
Whole fish |
|
|
Mini sampling (per fish) |
2.00
|
3.00 |
|
|
|
Standard sampling (per fish) |
3.60
|
5.40 |
|
|
|
Sampling kit (plus freight) |
15.00
|
27.00 |
|
|
|
Molecular
Diagnostics |
in state |
out of state |
|
|
|
Organism identification by PCR |
|
|
tissue, culture media |
|
|
Yersinia ruckeri
|
20.00
|
30.00 |
|
|
|
Flavobacterium psychrophilum |
35.00
|
52.50 |
|
|
|
Renibacterium salmoninarum (BKD) |
55.00 |
82.50 |
|
|
|
Mycobacterium spp./Fungal speciation |
|
|
|
|
|
Direct PCR/sequencing |
80.00 |
120.00 |
|
|
|
Cloning/sequencing |
150.00
|
225.00 |
|
|
|
Toxicology |
in state |
out of state |
|
|
|
Water quality analysis (per test) (ammonia, nitrite,
hardness, pH, alkalinity, chlorine) |
15.00 |
22.50 |
water |
|
|
For the evaluation of additional chemicals or substances
please contact WADDL
|
