College of Veterinary Medicine

Washington Animal Disease Diagnostic Lab

Aquatic Health


The Aquatic Health Section of WADDL provides health screening and diagnostic testing for marine and fresh water fish, including ornamental species. WADDL is accredited by the AAVLD and the Aquatic Health Section is USDA approved for testing live fish products for export. AFS and Canadian Health Inspectors on staff are available to assist with testing for interstate and international movement of live fish products.

General Policies
Bacteriology
Virology
Molecular Diagnostics
Pathology
Parasitology
Toxicology

 Aquaculture 2012  
   

General Policies

  1. All cases (except those exclusively for toxicology) will be assessed an accession fee of $10.00. An accession is defined as specimen(s) of one animal species or miscellaneous substances belonging to one owner and submitted at one time.
  2. Clinical pathology services (e.g., hematology and cytology) are provided by the Department of Veterinary Clinical Sciences (VCS) (509-335-0745). These services are billed by VCS.
  3. A FAX, mail or electronic report is provided at no charge.
  4. Samples sent for analysis to outside labs are billed for any additional direct costs incurred, including a handling fee (includes postage).
  5. Fees are subject to change without notice.
     

 Shipping address:

155N Bustad Hall
 Pullman, WA 99164-7034

Aquatic Health Inspection Testing

Aquatic animal health testing is performed in accordance with the guidelines outlined in both the OIE Manual of Diagnostic Tests for Aquatic Animals and the AFS-USFWS Blue Book.

Turn around time for all health inspections is 29 days based on the requirements set forth in both manuals for virology testing.

Two to four weeks advanced notice for scheduling purposes is strongly recommended on all health inspection samplings

 

Aquatic Health - AFS-USFWS Blue Book/OIE Health Inspection Testing

Test  Fees Specimen Type Comments
   in state out of state    
 Culture Identification of specific bacteria (per fish) 2.20 3.30  Kidney streaks on TSA, BHI or Blood Agar plates  Maintain samples below 15C
 Aerobic culture, contaminated plate surcharge (per tissue)  3.50  5.50    
Renibacterium salmoninarum (BKD)
      Culture (per fish)
      BKD FAT (per test)
      BKD ELISA (per test)
 9.50
 2.70
 5.25
 14.25
   4.05
   7.90		  Fresh Kidney or Ovarian Fluid  Maintain samples below 15C
Piscirickettsia salmonis Direct tissue touch exam
        Culture
        Touch Exam 
 21.00
   3.75
 31.50
   5.60		  Fresh Kidney  Maintain samples below 15C
M. cerebralis digest, small to medium sized fish (per test)  11.50 17.25  Head, skull section, gill arches  Maintain samples below 15C
M. cerebralis digest, large fish (per test)  14.00 21.00  Head, skull section, gill arches  Maintain samples below 15C
Ceratomyxa shasta by intestinal smear (per test) 3.75 5.65 Intestinal Mucosa  
Amoebic Gill Disease 8.00 12.00    
Bothriocephalus acheilognathi (Asian tapeworm) (per test) 3.75 5.65  Anterior 1/3 of intestine  Maintain samples below 15C
 Viral Culture (per test per cell line)

 Viral pathogens routinely tested for include:

 Epizootic hematopoietic necrosis virus
 Infectious hematopoietic necrosis virus
 Infectious pancreatic necrosis virus
 Oncorhynchus masou virus
 Viral hemorrhagic septicemia virus
 Infectious salmon anemia virus
 Spring Viremia of Carp
 Largemouth bass virus

  12.50
  18.75
  Maintain samples below 10C
 VHSV testing only (EPC cells)  18.00  27.00    
 Cell lines in active culture include:

 Epithelioma Papulosum Cyprini (EPC)
 Chinook Salmon Embryo (CHSE-214)
 Salmon Head Kidney (SHK-1)
 Fat Head Minnow (FHM)
 Bluegill Fry (BF-2)

 Contact lab for additional pathogens and/or
 cell lines

















   
 

 Aquatic Diagnostic Testing  
 Test Fees Specimen Type Comments
Bacteriology  in state out of state    
 Aerobic culture (per tissue)  9.50



 14.20  Fresh kidney, spleen, liver, brain; bacterial swab; streaked TSA, BHI or Blood Agar plates  Maintain samples below 15C
 Anerobic culture (per tissue)  10.50



 		  15.80



  Fresh kidney, spleen, liver, brain; bacterial swab; streaked TSA, BHI or Blood Agar plates  Maintain samples below 15C 
 Antimicrobial susceptibility  16.00 24.00    
 Fungal culture (genus)* 14.00 21.00    
 Mycobacterium (genus)* 17.50 26.25    
Virology  in state out of state    
 Viral isolation in cell culture (up to 4 cell lines)
 CHSE-214, EPC, SHK, FHM, BF-2		  70.00  105.00  Fresh kidney/spleen/pyloric caeca/gill filliment  Maintain samples below 10C
 Each additional cell line  12.50 18.75    
Parasitology  in state  out of state    
 External parasite screen on fresh tissues (per fish)  3.00  4.50  live fish  
Pathology  in state  out of state    
 Necropsy (includes gill wet prep/mucus-skin scrape)
      Fish 1 - 3
      Each additional fish
32.00
10.50
48.00
15.80		 live fish  
 Histopathology-Tissues from up to 3 fish
      Each additional fish		  32.00
10.50		  48.00
15.80 		 Formalin fixed whole fish, tissues  
 External parasite screen by Histopathology (per slide)  8.00 12.00  Formalin fixed whole fish, tissues  
Myxobolus cerebralis histopathology (per slide) 8.00 12.00  Formalin fixed head, skull section, gills  
 Sample collection at WADDL  in state  out of state  Whole fish  
 Mini sampling (per fish)  2.00  3.00    
 Standard sampling (per fish)  4.00  6.00    
 Sampling kit (plus freight)  20.00 30.00    
Molecular Diagnostics  in state  out of state    
 Organism identification by PCR      tissue, culture media  
Yersinia ruckeri 36.80 55.20    
Flavobacterium psychrophilum (less than 4 samples) 36.80 55.20    
     4 or more samples  25.00 37.50    
Renibacterium salmoninarum (BKD) 36.80 55.20    
Renibacterium salmoninarum (BKD) Nested 26.30 39.45    
 Spring viremia of carp virus (SVCV) 36.80 55.20    
Mycobacterium spp./Fungal speciation 100.00 150.00    
 Direct PCR/sequencing 100.00 150.00    
 Cloning/sequencing 200.00 300.00    

 Washington Animal Disease Diagnostic Laboratory
 Bustad Hall Room 155N
 Pullman WA 99164-7034

 (509) 335-9696, FAX (509) 335-7424

 e-mail: waddl@vetmed.wsu.edu
Last Edited: Apr 16, 2012 3:36 PM   


Washington Animal Disease Diagnostic Lab. PO Box 647034 , Washington State University, Pullman WA 99164-7010, 509-335-9696, Contact Us  Safety Links