Aquatic animal health testing is performed in accordance with
the guidelines outlined in both the OIE Manual of Diagnostic
Tests for Aquatic Animals and the AFS-USFWS Blue Book.
Turn around time for all health inspections is 29 days based
on the requirements set forth in both manuals for virology
testing.
Two to four weeks advanced notice for scheduling purposes is
strongly recommended on all health inspection samplings
|
Test |
Fees |
Specimen Type |
Comments |
|
|
in state |
out of state |
|
|
| Culture Identification of specific
bacteria (per fish) |
2.00 |
3.00 |
Kidney streaks on TSA, BHI or Blood Agar plates |
Maintain samples below 15ºC |
| Aerobic
culture, contaminated plate surcharge (per tissue) |
3.50 |
5.25 |
|
|
Renibacterium salmoninarum (BKD)
Culture (per fish)
BKD FAT (per test)
BKD ELISA (per test) |
9.00
2.50
5.00 |
13.50
3.75
7.50 |
Fresh
Kidney or Ovarian Fluid |
Maintain samples below 15ºC |
Piscirickettsia salmonis Direct tissue touch exam
Culture
Touch Exam |
20.00
3.50 |
30.00
5.25 |
Fresh
Kidney |
Maintain samples below 15ºC |
|
M. cerebralis digest, small to medium sized fish
(per test) |
11.00 |
16.50 |
Head,
skull section, gill arches |
Maintain samples below 15ºC |
|
M. cerebralis digest, large fish (per test) |
13.00 |
19.50 |
Head,
skull section, gill arches |
Maintain samples below 15ºC |
|
Ceratomyxa shasta by intestinal smear (per test) |
3.50 |
5.25 |
Intestinal Mucosa |
|
|
Bothriocephalus acheilognathi (Asian tapeworm)
(per test) |
3.50 |
5.25 |
Anterior 1/3 of intestine |
Maintain samples below 15ºC |
Viral Culture (per test per cell line)
Viral pathogens routinely tested for include:
Epizootic hematopoietic necrosis virus
Infectious
hematopoietic necrosis virus
Infectious pancreatic
necrosis virus
Oncorhynchus masou virus
Viral
hemorrhagic septicemia virus
Infectious salmon
anemia virus
Spring Viremia of Carp
Largemouth
bass virus |
12.00
|
18.00
|
|
Maintain samples below 10ºC |
| VHSV testing
only (EPC cells) |
17.00 |
25.50 |
|
|
Cell lines in
active culture include:
Epithelioma Papulosum Cyprini (EPC)
Chinook
Salmon Embryo (CHSE-214)
Salmon Head Kidney
(SHK-1)
Fat Head Minnow (FHM)
Bluegill Fry
(BF-2)
Contact lab for additional pathogens and/or
cell lines |
|
|
|
|
Aquatic Diagnostic Testing
|
|
Test |
Fees |
Specimen Type |
Comments |
|
Bacteriology |
in state |
out of state |
|
|
| Aerobic
culture (per tissue) |
9.00
|
13.50
|
Fresh
kidney, spleen, liver, brain; bacterial swab; streaked
TSA, BHI or Blood Agar plates |
Maintain samples below 15ºC |
| Anerobic
culture (per tissue) |
10.00
|
15.00
|
Fresh
kidney, spleen, liver, brain; bacterial swab; streaked
TSA, BHI or Blood Agar plates |
Maintain samples below 15ºC |
| Antimicrobial
susceptibility |
15.00 |
22.50 |
|
|
| Fungal
culture (genus)* |
13.00 |
19.50 |
|
|
| Mycobacterium
(genus)* |
16.75 |
25.00 |
|
|
|
Virology |
in state |
out of state |
|
|
Viral
isolation in cell culture (up to 4 cell lines)
CHSE-214, EPC, SHK, FHM, BF-2 |
70.00 |
105.00 |
Fresh
kidney/spleen/pyloric caeca/gill filliment |
Maintain
samples below 10ºC |
| Each
additional cell line |
12.00 |
18.00 |
|
|
|
Parasitology |
in state |
out of state |
|
|
| External
parasite screen on fresh tissues (per fish) |
3.00 |
4.50 |
live fish |
|
|
Pathology |
in state |
out of state |
|
|
Necropsy
(includes gill wet prep/mucus-skin scrape)
Fish 1 - 3
Each additional fish |
30.00
10.00 |
45.00
15.00 |
live fish |
|
Histopathology-Tissues from up to 3 fish
Each
additional fish |
30.00
10.00 |
45.00
15.00 |
Formalin
fixed whole fish, tissues |
|
| External
parasite screen by Histopathology (per slide) |
7.50 |
11.25 |
Formalin
fixed whole fish, tissues |
|
|
Myxobolus cerebralis histopathology (per slide) |
7.50 |
11.25 |
Formalin
fixed head, skull section, gills |
|
|
Sample collection at WADDL |
in state |
out of state |
Whole fish |
|
| Mini sampling
(per fish) |
2.00 |
3.00 |
|
|
| Standard
sampling (per fish) |
4.00 |
6.00 |
|
|
| Sampling kit
(plus freight) |
15.00 |
22.50 |
|
|
|
Molecular Diagnostics |
in state |
out of state |
|
|
| Organism
identification by PCR |
|
|
tissue,
culture media |
|
|
Yersinia ruckeri
|
30.00 |
45.00 |
|
|
|
Flavobacterium psychrophilum |
30.00 |
45.00 |
|
|
|
Renibacterium salmoninarum (BKD) |
35.00 |
52.50 |
|
|
|
Renibacterium salmoninarum (BKD) Nested |
25.00 |
37.50 |
|
|
| Spring
viremia of carp virus (SVCV) |
35.00 |
52.50 |
|
|
|
Mycobacterium spp./Fungal speciation |
84.00 |
126.00 |
|
|
| Direct
PCR/sequencing |
84.00 |
126.00 |
|
|
|
Cloning/sequencing |
165.00 |
247.50 |
|
|
