College of Veterinary Medicine

Washington Animal Disease Diagnostic Lab

Aquatic Health


The Aquatic Health Section of WADDL provides health screening and diagnostic testing for marine and fresh water fish, including ornamental species. WADDL is accredited by the AAVLD and the Aquatic Health Section is USDA approved for testing live fish products for export. AFS and Canadian Health Inspectors on staff are available to assist with testing for interstate and international movement of live fish products.

General Policies
Bacteriology
Virology
Molecular Diagnostics
Pathology
Parasitology
Toxicology

 
   

General Policies

  1. All cases except those exclusively for toxicology will be assessed an accession fee of $10.00. An accession is defined as specimen(s) of one animal species or miscellaneous substances belonging to one owner and submitted at one time.
     
  2. Clinical pathology services (e.g., hematology and cytology) are provided by the Department of Veterinary Clinical Sciences (VCS) (509-335-0745). These services are billed by VCS.
     
  3. A FAX, mail or electronic report is provided at no charge.
     
  4. Samples sent for analysis to outside labs are billed for any additional direct costs incurred. Handling fee (includes postage) is $15.00.
     
  5. Fees are subject to change without notice.
     
  6. Use Federal Express Account #2112-4551-4 for discounted shipping. Freight costs will be invoiced with case charges. FOR WADDL SUBMISSIONS ONLY.


Shipping address

155N Bustad Hall
Pullman, WA 99164-7034


Aquatic Health Inspection Testing

Aquatic animal health testing is performed in accordance with the guidelines outlined in both the OIE Manual of Diagnostic Tests for Aquatic Animals and the AFS-USFWS Blue Book.

Turn around time for all health inspections is 29 days based on the requirements set forth in both manuals for virology testing.

Two to four weeks advanced notice for scheduling purposes is stongly recommended on all health inspection samplings

Test Fees
Specimen Type Comments
Culture Identification of Aeromonas salmonicida and Yersinia ruckeri (per fish) 1.50 2.70 Kidney streaks on TSA, BHI or Blood Agar plates Maintain samples below 15ºC
Aerobic culture, contaminated plate surcharge (per tissue) 3.00 5.40    
Renibacterium salmoninarum (BKD)
     BKD FAT (per test)
     BKD ELISA (per test)

2.40
4.50
3.60
8.10
Fresh Kidney or Ovarian Fluid Maintain samples below 15ºC
Piscirickettsia salmonis Direct tissue touch exam 3.00 4.50 Fresh Kidney Maintain samples below 15ºC
M. cerebralis digest, small to medium sized fish (per test) 8.00 14.40 Head, skull section, gill arches Maintain samples below 15ºC
M. cerebralis digest, large fish (per test) 10.00 18.00 Intestinal Mucosa Maintain samples below 15ºC
Ceratomyxa shasta by intestinal smear (per test) 3.00 5.00    
Bothriocephalus acheilognathi (Asian tapeworm) (per test) 3.00 5.00 Anterior 1/3 of intestine Maintain samples below 15ºC
Viral Culture (per test per cell line)

Viral pathogens routinely tested for include:

Epizootic hematopoietic necrosis virus
Infectious hematopoietic necrosis virus
Infectious pancreatic necrosis virus
Oncorhynchus masou virus
Viral hemorrhagic septicemia virus
Infectious salmon anemia virus
Spring Viremia of Carp
(Contact lab regarding additional pathogens)

10.50 17.00    
 

Aquatic Diagnostic Testing  

Test Fees Specimen Type Comments
Bacteriology in state out of state    
Aerobic culture (per tissue) 7.00 10.50 Fresh kidney, spleen, liver, brain; bacterial swab; streaked TSA, BHI or Blood Agar plates Maintain samples below 15ºC
Anerobic culture (per tissue) 6.50 9.75 Fresh kidney, spleen, liver, brain; bacterial swab; streaked TSA, BHI or Blood Agar plates Maintain samples below 15ºC 
Antimicrobial susceptibility 12.00 15.00    
Renibacterium salmoninarum (BKD) culture (per test) 8.50 15.30 Fresh Kidney Maintain samples below 15ºC
Piscirickettsia salmonis culture (per test) 20.00 30.00 Fresh Kidney Maintain samples below 15ºC
Fungal culture (genus)* 11.00 15.75    
Mycobacterium (genus)* 15.75 22.50    
*For speciation see molecular diagnostics
Virology in state out of state    
Viral isolation in cell culture (up to 4 cell lines) 70.00 105.00 Fresh kidney/spleen/pyloric caeca/gill filliment Maintain samples below 15ºC
Each additional cell line        
Cell lines in active culture include:

Epithelioma Papulosum Cyprini (EPC)
Chinook Salmon Embryo (CHSE-214)
Salmon Head Kidney (SHK-1)
Fat Head Minnow (FHM)
Bluegill Fry (BF-2)

For questions regarding cell lines not listed, please contact WADDL

Parasitology in state out of state    
External parasite screen on fresh tissues (per fish) 2.40 3.60 live fish  
Pathology in state out of state    
Necropsy (includes gill wet prep/mucus-skin scrape)
     Fish 1 -5
     Each additional fish

 

25.00
  5.00


37.50
7.50

 

live fish  
Histopathology-Tissues from up to 5 fish
     Each additional fish
25.00
10.00
37.50
15.00
Formalin fixed whole fish, tissues  
External parasite screen by Histopathology (per slide) 5.00 9.00 Formalin fixed whole fish, tissues  
Myxobolus cerebralis histopathology (per slide) 5.00 9.00 Formalin fixed head, skull section, gills  
Sample collection at WADDL in state out of state Whole fish  
Mini sampling (per fish) 2.00 3.00    
Standard sampling (per fish) 3.60 5.40    
Sampling kit (plus freight) 15.00 27.00    
Molecular Diagnostics in state out of state    
Organism identification by PCR     tissue, culture media  
Yersinia ruckeri 20.00 30.00    
Flavobacterium psychrophilum 35.00 52.50    
Renibacterium salmoninarum (BKD) 55.00 82.50    
Mycobacterium spp./Fungal speciation        
Direct PCR/sequencing 80.00 120.00    
Cloning/sequencing 150.00 225.00    
Toxicology in state out of state    
Water quality analysis (per test) (ammonia, nitrite, hardness, pH, alkalinity, chlorine) 15.00 22.50 water  
For the evaluation of additional chemicals or substances please contact WADDL

Washington Animal Disease Diagnostic Laboratory
PO Box 2037 College Station
Pullman, WA 99165-2037

(509) 335-9696, FAX (509) 335-7424

e-mail: waddl@vetmed.wsu.edu


Last Edited: May 04, 2007 5:01 PM
Washington Animal Disease Diagnostic Lab. PO Box 647034 , Washington State University, Pullman WA 99164-7010, 509-335-9696, Contact Us  Safety Links