College of Veterinary Medicine

Washington Animal Disease Diagnostic Lab

Avian Health & Food Safety Laboratory


General Policies
Necropsy
Submitting birds for Necropsy
Serology
Bacteriology
Virology
Parasitology
Miscellaneous
Food Safety
PCR Tests
Toxicology
Forms
Faculty & Staff

Shipping address:
2607 W Pioneer
Puyallup, WA 98371-4990
Phone: 253-445-4537 


Puyallup Research and Extension Center

 
 

 Lynn's Hummer
Avian Lab
Give to the Avian Health Laboratory Endowment
   

  Non-O157 STEC Testing at AHFSL

Because of the public health concern regarding the non-O157 Shiga Toxin-producing Escherichia coli (STEC) serogroups, FSIS has declared six additional E. coli strains (O26, O45, O103, O111, O121, and O145) as adulterants in food (http://www.fsis.usda.gov/OPPDE/rdad/FRPubs/2010-0023.pdf ). This regulation places these non-O157 STEC strains in the same regulatory category as the much-feared E. coli O157:H7. Declaring a substance to be anadulterant means that it cannot legally be distributed in food, and therefore food processors can legally be held accountable for products that contain it.
Starting on June 4, 2012, beef manufacturing trimmings are required to be tested for these bacteria. At this time, the rule applies only to raw ground beef and in the scraps or “trim” that are collected in processing plants, under the MT60, M52, and MT53 sampling programs, to be made into ground beef. The rule does not apply to components of ground beef, such as cheek meat, head meat, or other components described in FSIS Directive 10,010.1.
The Avian Health and Food Safety laboratory now offers a Real-Time STEC assay for the rapid screening of the virulence genes present in STEC serotypes. Furthermore, if a positive sample is detected, we will analyze for the presence or absence of the O group. Contact the lab at 253-445-4537 or email WADDLAHL@vetmed.wsu.edu  for a quote.

General Policies

  1. All cases except those exclusively for toxicology and food safety will be assessed an accession fee of $10.00. An accession is defined as specimen(s) of one animal species or miscellaneous substances belonging to one owner and submitted at one time.
  2. Non-Washington samples will be assessed a ~50% surcharge except where indicated.
  3. A FAX, mail, or electronic report is provided at no charge.
  4. Samples sent for analysis to outside labs are billed for any additional direct costs incurred plus a handling fee (includes postage) is $20.00.
  5. Fees are subject to change without notice.  Current fees are located on the WADDL Web site at www.vetmed.wsu.edu/depts_waddl.
  6. "STAT" fee - Please contact laboratory in advance to arrange scheduling and fees.
Administration
  in state out of state
Accession fee 10.00 10.00
Outside lab handling fee 20.00 20.00
Fed Ex delivery charge (under 3 lbs.) 10.00 18.00
Fed Ex delivery charge (over 3 lbs.) actual rate actual rate
Testing for export 5.00 7.00
     

 Necropsy
  in state out of state
Gross only (birds) 11.60 17.30
Cosmetic necropsy (under 5 pounds) 23.10 34.70
Histo on AHFSL necropsy 18.90 28.40
Duplicate slide 6.30 9.50

      

Immunodiagnostics/Serology
ELISA in state out of state
Infectious Bronchitis (IB) 1.60 2.40
Infectious Bursal Disease (IBDV) 1.60 2.40
Newcastle Disease (NDV) 1.60 2.40
Reovirus infection – viral arthritis (REO) 1.60 2.40
Pseudorabies (IgB & IgI)* 4.00  
     *USDA-APHIS Program testing    
AGID (Agar Gel Immunodiffusion) in state out of state
Adenovirus Infection (ADENO) 2.00 3.00
Avian Encephalomyelitis (AE) 2.00 3.00
Avian Influenza (AI) - from serum (blood) 2.00 3.00
Avian Influenza (AI)  - from yolk (egg) 7.00 10.50
Infectious Bursal Disease – Gumboro (IBDV) 2.00 3.00
Reovirus Infection (REO) 2.00 3.00
HI (Hemagglutination Inhibition) in state out of state
Infectious Bronchitis (IB) 2.30 3.50
Mycoplasma gallisepticum (MG) 2.30 3.50
Mycoplasma synoviae (MS) 2.30 3.50
Newcastle Disease (NDV) 2.30 3.50
Agglutination in state out of state
Plate Agglutination (MG, MS, MM, S. Pullorum/typhoid) .80 1.20
Microtiter confirmation for positive agglutination 1.10 1.60
Brucellosis* in state out of state
(BAPA, Rivanol, Complement Fixation) 4.20 6.30
     *Species tested are cattle, goats, and cervids    

Bacteriology
  in state out of state
Aerobic 3.50 5.20
Anaerobic 5.80 8.70
Mycology (Aspergillus, other fungus) 5.80 8.70
Salmonella (from tissue) 10.00 15.00
Salmonella  (from drag swabs), FDA and NPIP methods 25.00 35.00
Salmonella (from eggs), FDA method 35.00 52.50
Salmonella serogrouping 7.00 10.50
Antibiotic Sensitivity 9.20 13.90

Virology
  in state out of state
Virus isolation (SPF embryos, includes up to 3 passages) 100.00 150.00

Parasitology
  in state out of state
Fecal Float 6.30 9.50
Coccidiosis evaluation 6.30 9.50

Miscellaneous
Call the lab for price and availability of drag swabs, sponges, culturettes, sexing cards, and other materials.    

Food Safety
No accession charge for food safety in state out of state
Molecular Methods (pricing includes confirmation)
Samonella sp. 30.00 45.00
Listeria sp. 30.00 45.00
Listeria monocytogenes 30.00 45.00
Cultural Methods    
Aerobic plate count, food 11.60 17.30
Anaerobic plate count, food 20.00 30.00
Bacillus cereus 23.10 34.70
Bacterial ID 26.25 39.40
Campylobacter sp. 22.30 33.40
Coliform count 9.50 14.20
High Sensitivity Coliform count 10.50 15.80
Enterobacter 17.30 26.00
E.coli, O157:H7 28.90 43.30
E.coli, O157:H7 - Robust procedure 57.80 86.60
E.coli, O157:H7 - Robust procedure and non-O157 STEC 92.80 143.70
Listeria sp. culture 22.30 33.40
Moisture determination, food product 17.30 26.00
Salmonella sp. 20.00 30.00
Salmonella for egg product - PERP program 23.10 34.70
Salmonella (MPN procedure) 52.00 78.00
Staphylococcus aureus 17.30 26.00
Yeast/Mold 17.30 26.00
Confirmation tests for cultural methods    
Campylobacter sp. 7.70 11.60
E. coli O157 27.00 45.00
Listeria sp. confirmation/id 7.70 11.60
Salmonella serogrouping 7.00 10.50
Analytical tests    
pH of food sample 14.00 21.00
Water Activity 22.00 33.00

     

PCR Tests
PCR Tests for Avian Pathogens in state out of state
Avian influenza - Individual* 40.00  
Avian Influenza - pooling* 45.00  
Avian paramyxovirus (Newcastle's Disease)* 40.00  
Avian paramyxovirus (Newcastle's Disease) - pooling* 45.00  
Chicken Anemia Virus (less than 4) 36.80 55.20
     4 or more samples  25.00 37.50
Chlamydiophila psittaci 36.80 55.20
Circo Virus (pigeon) 36.80 55.20
Infectious Bronchitis Virus (less than 4 samples) 36.80 55.20
     4 or more samples  25.00 37.50
Infectious Laryngotracheitis Virus (ILT) (less than 4 samples) 36.80 55.20
     4 or more samples  25.00 37.50
Mycoplasma spp., M. gallisepticum, M> synoviae (less than 4 samples) 36.80 55.20
    4 or more samples  25.00 37.50
Pacheco’s Disease Herpesvirus 36.80 55.20
Polyoma Virus (psittacine) 36.80 55.20
Psittacine Beak & Feather Disease (PBFD) 36.80 55.20
West Nile Virus 36.80 55.20
Other PCR Tests in state out of state
Avian Sex Determination 15.00 22.50
     *USDA-APHIS Program testing

Toxicology
Toxicology testing is conducted at the University of Idaho Analytical Sciences Laboratory. Additional analyses including comprehensive plant, water, soil and environmental analytic services are available. Inquiries concerning toxicology testing should be made directly to the toxicology laboratory (208) 885-7081.

Avian Health and Food Safety Laboratory
2607 W Pioneer
Puyallup, WA 98371-4990
(253) 445-4537, FAX (253) 445-4544

Last Edited: May 22, 2013 1:45 PM   


Washington Animal Disease Diagnostic Lab. PO Box 647034 , Washington State University, Pullman WA 99164-7010, 509-335-9696, Contact Us  Safety Links