| |
I have
also helped with an Enterococcus bead array, which will be able to
help identify where an isolate came from, i.e. cattle, humans, etc.
While in this lab, I have also done microarray with Flavobacterium
species and bead arrays with IHNV, IPNV, and VHSV.Before becoming a
part of Dr. Call’s lab at Washington State University, I worked at
the University of Idaho in Dr. Carolyn Bohach’s lab trying to
identify E. coli O157 colonization in cattle, acid resistance of E.
coli O157 in the gastrointestinal tract of cattle, and vaccine
trials against the pathogen in cattle.
Education
B.S.
Colorado State University 2000-2001, Zoology
Iowa State University 1997-2000, Zoology
Professional Positions
Research Associate November, 2002 - Present
Department of Veterinary Microbiology and Pathology
College of Veterinary Medicine
Washington State University
Research Assistant June, 2001 - November, 20002
University of Idaho
Skills
Printing microarrays
Coupling PCR products to microspheres
DNA and RNA extraction
Nick translation
DNA hybridization
PCR
RT-PCR
Production of probe constructs
Bacterial culture
Restriction enzyme digests
Publications
Soule, M., LaFrentz, S., Cain, K., LaPatra, S.,
and Call, D.R. 2005. Polymorphisms in 16s rRNA genes of
Flavobacterium psychrophilum correlate with elastin hydrolysis and
tetracycline resistance. Diseases of Aquatic Organisms 65:209-216.
Soule, M., Cain, K., LaFrentz, S., and Call, D.R. 2005. Combining
suppression subtractive hybridization and microarrays to map the
intra-specific phylogeny of Flavobacterium psychrophilum. Infection
and Immunity 73:3799-3802.
Warsen, A.E., Krug, M.J., LaFrentz, S.A., Stanek, D.R., Loge, F.J.,
Call, D.R. 2004. Simultaneous discrimination between 15 fish
pathogens using 16S rDNA PCR and DNA microarrays. Applied and
Environmental Microbiology 70:4216-4221.
Rice, D.H., Sheng, H.Q., Wynia, S.A., Hovde, C.J. 2003. Rectoanal
mucosal swab culture is more sensitive than fecal culture and
distinguishes Escherichia coli O157:H7-colonized cattle and those
transiently shedding the same organism. Journal of Clinical
Microbiology 41, 4924-4929.
Grauke, L.J., Wynia, S.A., Sheng, H.Q., Yoon, J.W., Williams, C.J.,
Hunt, C.W., Hovde, C.J. 2003. Acid resistance of Escherichia coli
0157:H7 from the gastrointestinal tract of cattle fed hay or grain.
Veterinary Microbiology 95, 211-225.
Presentations and Posters
Soule, LaFrentz, Oatley, Krug, Cain, and Call. 2004. “Virulent and
avirulent strains of Flavobacterium psychrophilum compared using
suppression subtractive hybridization”. Poster (Abstract accepted).
AAAS Annual Meeting. Seattle, WA, U.S.A.
Soule, LaFrentz, Oatley, Krug, LaFrentz, Cain and Call. May 2004.
Comparative genetics of virulent and avirulent strains of
Flavobacterium psychrophilum, etiological agent for cold water
disease in salmonids. Poster. ASM Annual meeting. New Orleans, LA,
U.S.A.
Soule, LaFrentz, Oatley, Krug, LaFrentz, Cain and Call. June 2004.
Comparative genetics of virulent and avirulent strains of
Flavobacterium psychrophilum, etiological agent for cold water
disease in salmonids. Poster. WSU and UI Center for Reproductive
Biology, Annual Retreat. ID, U.S.A.
Warsen A., Stanek, D., Krug, M.J., LaFrentz, S.A., Loge, F.J., Call,
D.R. June 2004. Pathogen detection using 16S PCR and DNA microarrays.
WSU and UI Center for Reproductive Biology, Annual Retreat. ID,
U.S.A.
Soule, LaFrentz, Oatley, Krug, LaFrentz, Cain and Call. 3 Sep 2004.
Comparative genetics of virulent and avirulent strains of
Flavobacterium psychrophilum, etiological agent for cold water
disease in salmonids. Poster. Center for Integrated Biotechnology
Retreat. Moscow, ID, U.S.A.
Soule, Cain, LaFrentz, and Call. 8 Sept. 2004. Virulent and
avirulent Flavobacterium psychrophilum strains compared using
suppression subtractive hybridization and microarrays. Oral
presentation. Bacterial Coldwater Disease Working Group FLAVO
meeting. Seattle, WA, U.S.A.
Soule, LaFrentz, Oatley, Krug, LaFrentz, Cain and Call. 14 Oct.
2004. Comparative genetics of virulent and avirulent strains of
Flavobacterium psychrophilum, etiological agent for cold water
disease in salmonids. 6th Annual College of Veterninary Medicine
Research Symposium. Washington State University, Pullman, WA, U.S.A.
Warsen A., Stanek, D., Krug, M.J., LaFrentz, S.A., Loge, F.J., Call,
D.R. 2003. Pathogen detection using 16S PCR and DNA microarrays.
College of Veterinary Medicine Symposium, Washington State
University, Pullman, WA, U.S.A.
Call, D.R., Soule, M., Oatley, M.J., Krug, M.J., LaFrentz, S.A.,
Loge, F.J., Gay. 2003. Identifying host-specific markers of fecal
pollution using mixed-genome microarrays. WSU Center for Integrated
Biotechnology, First Annual Retreat, September 11-12, Moscow, ID,
U.S.A.
Soule, M., LaFrentz, S.A., Oatley, M.J., Krug, M.J., Cain, K.D.,
Call D.R. 2003. Virulent and avirulent strains of Flavobacterium
psychrophilum compared using suppression subtractive hybridization.
WSU Center for Integrated Biotechnology, First Annual Retreat,
September 11-12, Moscow, ID, U.S.A.
Staff List
|
ZRU Home
| Revised
March 07, 2006
|
Printer Friendly Version
Contact
us: webmaster@wsu.edu
509-335-9515 | Accessibility
| Copyright | Policies
College of Veterinary Medicine, PO Box 647010, Washington
State University, Pullman, WA 99164-7010 USA
Emergency
Preparedness & Safety Links
|
|
