Caroline Engle Distinguished Professor of Research on
Infectious Disease
Paul G. Allen School for Global Animal Health
Adjunct Faculty in the Department of Electrical
Engineering and Computer Science
B.S. Washington State University, 1983, Wildlife Management
M.S. Humboldt State University, 1990, Wildlife Management
Ph.D. Washington State University, 1997, Zoology
Postdoctoral Fellow in Immunopathology, University of Michigan, 1997-1999
Postdoctoral Fellow in Environmental Microbiology, Pacific Northwest
National Laboratory, 1999
My lab is engaged in research involving comparative genomics, transcriptomics, and proteomics. Current research topics include:
Antibiotic resistance: Since 2000 my lab has studied factors that drive the emergence and maintenance of antibiotic resistance in the absence of antibiotic use with an emphasis on enteric bacteria of cattle. Our current work is focused on multidrug resistance plasmids that that carry
blaCMY-2, which is a gene that encodes resistance to third-generation cephalosporins. Our work examines (1) the fitness costs of carrying this plasmid in
E. coli and Salmonella enterica, (2) segregation control at cell division, (3) mobilization of plasmid-encoded transposons to new plasmids and mobilization of the plasmids to new host cells, and (4) transcriptional control of plasmid encoded genes. We are also engaged in studies that examine the source, fate, and transport of antibiotics in the environment with a special focus on the biological consequences of these drugs and the subsequent impact on public and animal health and welfare.
Type III secretion systems (T3SS): A T3SS is a needle-like apparatus that pathogenic bacteria (Gram negative) use to inject bacterial proteins (effectors) into host cells where the proteins can interrupt cell division, change cell morphology and behavior, and induce cell death through a variety of mechanisms. We are particularly interested in identifying effector proteins and determining their biological function with a focus on
Vibrio parahaemolyticus. We are also engaged in studies of the transcriptional regulation of T3SS genes and in assessing the biological relevance of T3SS effector proteins using animal models.
Bacterial diseases of salmonid aquaculture: Bacterial coldwater disease, caused by
Flavobacterium psychrophilum, is the leading cause bacterial disease losses to salmonid aquaculture in the Pacific Northwest. My lab has helped delineate the population genetic structure of this pathogen and we are actively engaged in efforts to develop vaccines and diagnostics to prevent and control this important disease. My lab is also engaged in research to identify the etiologic agent of strawberry disease in rainbow trout for which we have identified a rickettsial-like organism as the probable agent.
Other projects: My lab is involved in continuing work on a variety of projects involving other important pathogens (Campylobacter jejuni;
S. enterica serovars Typhimurium, Newport, Enteritidis, and Dublin; norovirus; and
Listeria monocytogenes) and my group is involved in collaborative work in environmental microbiology, water quality, pathogen detection, protein expression, and molecular epidemiology.
For more details about the scope of our work, please visit our lab webpage:
http://www.vetmed.wsu.edu/research_vmp/MicroArrayLab/
Call, DR, L Orfe, MA Davis, S LaFrentz and MS
Kang. 2008. Impact of compounding error on strategies for subtyping pathogenic bacteria. Foodborne Pathogens and Disease 5:505-516. PMID: 18713065.
Call, DR, MA Davis, and AA Sawant. 2008. Antimicrobial resistance in beef and dairy cattle production. Animal Health Research Reviews 9:159-167. PMID: 18983724.
Call, DR and P Plescia. 2008. Identifying sources of fecal pollution in the Colville River using library-independent genetic markers. Northwest Science 82:120-127.
Chen, J, MA Davis, SE LaPatra, K Cain, K Snekvik, and DR Call. 2008. Genetic diversity of Flavobacterium psychrophilum recovered from commercially raised rainbow trout and spawning Coho salmon. Journal of Fish Diseases 31:765-773.
Daniels,
JB, DR Call, DD Hancock, WM Sischo, K Baker, and TE Besser. 2009. The
role of ceftiofur in the selection and dissemination of
blaCMY-2 – mediated
cephalosporin resistance in
Salmonella enterica and
commensal Escherichia coli
isolated from cattle. Applied and Environmental Microbiology 75:3648-3655.
PMID: 19376926.
Davis, MA, KNK Baker, DR Call, LD Warnick, Y Soyer, M Wiedmann, Y
Gröhn, PL McDonough, DD Hancock, and TE Besser. 2009. Multiple locus
variable number of tandem repeats typing method for
Salmonella enterica serovar
Newport. Journal of Clinical Microbiology 47:1934-1938.
PMID: 19386855.
LaFrentz,
BR, SE LaPatra, DR Call, GD Wiens, and KD Cain. 2009. Proteomic
analysis of Flavobacterium
psychrophilum cultured
in vivo and in iron-limited
media. Diseases of Aquatic Organisms 87:171-182.
PMID: 20099411.
Lindstrom,
NM, DR Call, ML House, CM Moffitt, and KD Cain. 2009. A quantitative
enzyme-linked immunosorbent assay (ELISA) and filtration-based fluorescent
antibody test as potential tools for screening
Flavobacterium
psychrophilum in broodstock.
Journal of Aquatic Animal Health 21:43-56.
PMID: 19485125.
Stanton,
JB, DP Knowles, DR Call, BA Mathison, and TV Baszler. 2009. Limited
transcriptional response of ovine microglia to prion accumulation.
Biochemical and Biophysical Research Communications 386:345-350.
PMID: 19523453.
Zhou,
X, ME Konkel, and DR Call. 2009. Type III secretion system 1 of
Vibrio
parahaemolyticus induces oncosis
in both epithelial and monocytic cell lines. Microbiology 155:837-851.
PMID: 19246755.
Allen,
JG, MW Beutel, DR Call, and A Fischer. 2009. Effects of Oxygenation
on ammonia oxidation potential and microbial diversity in sediment from
surface-flow wetland mesocosms. Bioresource Technology
doi:10.1016/j.biotech.2009.09.050.
PMID: 19815408.
Call,
DR, RS Singer, D Meng, SL Broschat, LH Orfe, JM Anderson, DR Herndon,
LS Kappmeyer, JB Daniels, and TE Besser. 2010.
BlaCMY-2 positive Inc
A/C plasmids from Escherichia
coli and
Salmonella
enterica are a distinct component
of a larger lineage of plasmids. Antimicrobial Agents and Chemotherapy.
54:590-596. PMID: 19949054.
Dassanayake,
RP, DR Call, AA Sawant, NC Casavant, GC Weiser, DP Knowles, and S
Srikumaran. 2010. Bibersteinia
trehalosi inhibits growth of
Mannheimia haemolytica by a contact-dependent mechanism. Applied and
Environmental Microbiology 76:1008-1013. PMID: 20038698.
Davis, MA, KNK Baker, L Orfe, D Shah, TE Besser, and DR Call. 2010.
Discovery of a gene conferring high-level aminoglycoside resistance in
Escherichia coli. Antimicrobial
Agents and Chemotherapy 54:2666-2669. PMID: 20368404.
Zhou,
X, ME Konkel, and DR Call. 2010. Vp1659 is a
Vibrio
parahaemolyticus T3SS1 protein
that contributes to translocation of effector proteins needed to induce
cytolysis, autophagy and disruption of actin structure in HeLa cells.
Journal of Bacteriology 192:3491-3502. PMID: 20418402.
Zhou,
X, ME Konkel, and DR Call. 2010. Regulation of type III secretion
system 1 gene expression in Vibrio
parahaemolyticus is dependent on interactions between ExsA, ExsC, and
ExsD. Virulence 1:260-272.
Ramsay,
JD, SR Leib, L Orfe, DR Call, RL Tallmadge, DG Fraser, and RH Mealey.
2010. Development of a DNA microarray for detection of expressed equine
classical MCH class I alleles in a defined population. Immunogenetics
62:633-639. PMID: 20683590.
Okubara, PA, DR Call, Y Kwak, and DZ Skinner. 2010. Induction of
defense gene homologues in wheat roots during interactions with
Pseudomonas
fluorescens. Biological Control
55:118-125.
Davis, MA, JY Lim, Y Soyer, H Harbottle, YF Chang, D New, L Orfe, TE Besser,
and DR Call. 2010. Development and validation of a resistance and
virulence gene microarray targeting
Escherichia coli and Salmonella
enterica: intra-laboratory validity v. inter-laboratory portability.
Journal of Microbiological Methods 82:36-41. PMID: 20362014.
Metselaar, M, KD Thompson, RML Gratacap, MJL Kik, SE LaPatra, SJ Lloyd,
DR Call, PD Smith, and A Adams. 2010. Association of red-mark
syndrome with a Rickettsia-like
organism and its connection with strawberry disease in the USA. Journal of
Fish Diseases 33:849-858. PMID: 20854353.
Piñeyro,
P, X Zhou, LH Orfe, PJ Friel, K Lahmers, and DR Call. 2010.
Development of animal models to study the function of
Vibrio
parahaemolyticus type III
secretion systems. Infection and Immunity 78:4551-4559. PMID: 20823199.
Broschat SL, DR Call, MA Davis, D Meng, A Ahmed, and TE Besser.
2010. Improved identification of epidemiologically related strains of
Salmonella
enterica using a fusion algorithm
based on PFGE and MLVA. Journal of Clinical Microbiology 48:4072-4082.
Straub, TM, RA Bartholomew, CO Valdez, NB Valentine, A Dohnalkova, RM
Ozanich, CJ Bruckner-Lea and DR Call.
In press. Human norovirus
infection of Caco-2 cells grown as a 3-dimensional tissue structure. Journal
of Water and Health.
Plant, KP, SE LaPatra, DR Call, and KD Cain.
In Press. Immunization of rainbow
trout (Oncorhynchus mykiss) with
Flavobacterium psychrophilum
proteins elongation factor-Tu, SufB Fe-S assembly protein and ATP synthaseb.
Journal of Fish Diseases.
Sawant, AA, NC Casavant, DR Call, and TE Besser. 2011. Identification
and characterization of proximity-dependent inhibition from
Escherichia
coli isolated from cattle.
Applied and Environmental Microbiology 77:2345-2351.
Shah, DH, X Zhou, T Addwebi, MA Davis, DR Call, J
Guard, and TE Besser. Accepted.
Cell invasion of poultry-associated
Salmonella Enteritidis isolates correlates with virulence, motility and
secretion of type-three secretion system effector proteins. Microbiology.
Lloyd,
SJ, SE LaPatra, KR Snekvik, KD Cain, and DR Call.
Accepted. Quantitative PCR
demonstrates a positive correlation between a
Rickettsia-like organism and
severity of strawberry disease lesions in rainbow trout (Oncorhynchus
mykiss). Journal of Fish
Diseases.
Davis, MA, TE Besser, LH Orfe, KNK Baker, AS Lanier, SL Broschat, D
New, and DR Call. Accepted.
Genotypic-phenotypic discrepancies between antibiotic resistance
characteristics of Escherichia coli
from calves in high and low antibiotic use management settings. Applied and
Environmental Microbiology.
Herndon,
CN, DR Call, TV Baszler, B Mathison, DP Knowles, and S Srikumaran.
Accepted. Comparison of passively
transferred antibodies in bighorn and domestic lambs reveals one factor in
differential susceptibility of these species to
Mannheimia
haemolytica-induced pneumonia.
Clinical and Vaccine Immunology.
Subbiah,
M, EM Top, DH Shah, and DR Call.
Accepted. The long-term
persistence of blaCMY-2
positive, IncA/C plasmids requires selection pressure. Applied and
Environmental Microbiology.
Shah, DH, X Zhou, T Addwebi, MA Davis and DR Call.
Accepted. Phenotypic diversity
among clinical strains of Salmonella
Enteritidis isolated from North America. Archives of Microbiology.
LaFrentz,
BR, SE LaPatra, DR Call, GD
Wiens, and KD Cain. Accepted.
Identification of immunogenic proteins of
Flavobacterium
psychrophilum as potential
vaccine candidates. Journal of Fish Diseases.
Righter,
Rurangirwa, Call, and McElwain.
Accepted. Development of a
bead-based multiplex PCR assay for the simultaneous detection of multiple
Mycoplasma species. Veterinary
Microbiology.
