B.S. Washington State University, 1983, Wildlife Management
M.S. Humboldt State University, 1990, Wildlife Management
Ph.D. Washington State University, 1997, Zoology
Postdoctoral Fellow in Immunopathology, University of Michigan, 1997-1999
Postdoctoral Fellow in Environmental Microbiology, Pacific Northwest
National Laboratory, 1999
My lab is engaged in research involving comparative genomics, transcriptomics, and proteomics. Current research topics include:
Antibiotic resistance: Since 2000 my lab has studied factors that drive the emergence and maintenance of antibiotic resistance in the absence of antibiotic use with an emphasis on enteric bacteria of cattle. Our current work is focused on multidrug resistance plasmids that that carry blaCMY-2, which is a gene that encodes resistance to third-generation cephalosporins. Our work examines (1) the fitness costs of carrying this plasmid in E. coli and Salmonella enterica, (2) segregation control at cell division, (3) mobilization of plasmid-encoded transposons to new plasmids and mobilization of the plasmids to new host cells, and (4) transcriptional control of plasmid encoded genes. We are also engaged in studies that examine the source, fate, and transport of antibiotics in the environment with a special focus on the biological consequences of these drugs and the subsequent impact on public and animal health and welfare.
Type III secretion systems (T3SS): A T3SS is a needle-like apparatus that pathogenic bacteria (Gram negative) use to inject bacterial proteins (“effectors”) into host cells where the proteins can interrupt cell division, change cell morphology and behavior, and induce cell death through a variety of mechanisms. We are particularly interested in identifying effector proteins and determining their biological function with a focus on Vibrio parahaemolyticus. We are also engaged in studies of the transcriptional regulation of T3SS genes and in assessing the biological relevance of T3SS effector proteins using animal models.
Bacterial diseases of salmonid aquaculture: Bacterial coldwater disease, caused by Flavobacterium psychrophilum, is the leading cause bacterial disease losses to salmonid aquaculture in the Pacific Northwest. My lab has helped delineate the population genetic structure of this pathogen and we are actively engaged in efforts to develop vaccines and diagnostics to prevent and control this important disease. My lab is also engaged in research to identify the etiologic agent of strawberry disease in rainbow trout for which we have identified a rickettsial-like organism as the probable agent.
Other projects: My lab is involved in continuing work on a variety of projects involving other important pathogens (Campylobacter jejuni; S. enterica serovars Typhimurium, Newport, Enteritidis, and Dublin; norovirus; and Listeria monocytogenes) and my group is involved in collaborative work in environmental microbiology, water quality, pathogen detection, protein expression, and molecular epidemiology.
For more details about the scope of our work, please visit our lab webpage:
http://www.vetmed.wsu.edu/research_vmp/MicroArrayLab/
Call, DR, L Orfe, MA Davis, S LaFrentz and MS Kang. 2008. Impact of compounding error on strategies for subtyping pathogenic bacteria. Foodborne Pathogens and Disease 5:505-516. PMID: 18713065.
Call, DR, MA Davis, and AA Sawant. 2008. Antimicrobial resistance in beef and dairy cattle production. Animal Health Research Reviews 9:159-167. PMID: 18983724.
Call, DR and P Plescia. 2008. Identifying sources of fecal pollution in the Colville River using library-independent genetic markers. Northwest Science 82:120-127.
Chen, J, MA Davis, SE LaPatra, K Cain, K Snekvik, and DR Call. 2008. Genetic diversity of Flavobacterium psychrophilum recovered from commercially raised rainbow trout and spawning Coho salmon. Journal of Fish Diseases 31:765-773.
Daniels, JB, DR Call, DD Hancock, WM Sischo, K Baker, and TE Besser. 2009. The role of ceftiofur in the selection and dissemination of blaCMY-2 – mediated cephalosporin resistance in Salmonella enterica and commensal Escherichia coli isolated from cattle. Applied and Environmental Microbiology 75:3648-3655.
Davis, MA, KNK Baker, DR Call, LD Warnick, Y Soyer, M Wiedmann, Y Gröhn, PL McDonough, DD Hancock, and TE Besser. 2009. Multiple locus variable number of tandem repeats typing method for Salmonella enterica serovar Newport. Journal of Clinical Microbiology 47:1934-1938.
Khachatryan, AR, TE Besser, and DR Call. 2008. The SSuT antimicrobial resistance element form calf-adapted Escherichia coli is widely distributed in Washington State cattle. Applied and Environmental Microbiology 74:391-395.
LaFrentz, BR, SE LaPatra, DR Call, and KD Cain. 2008. Development and characterization of rifampicin resistant Flavobacterium psychrophilum strains and their potential as live attenuated vaccine candidates. Vaccine 26:5582-5589. PMID: 18708112.
LaFrentz, BR, SE LaPatra, DR Call, GD Wiens, and KD Cain. In press. Proteomic analysis of Flavobacterium psychrophilum cultured in vivo and in iron-limited media. Diseases of Aquatic Organisms.
Larson, CL, DH Shah, AS Dhillon, DR Call, S Ahn, GJ Haldorson, C Davitt, and ME Konkel. 2008. Campylobacter jejuni invades chicken LMH cells inefficiently and stimulate differential expression of the chicken CXCLi1 and CXCLi2 cytokines. Microbiology 154:3835-3847. PMID: 19047751.
Leach, MD, SL Broschat, and DR Call. 2008. A discrete, stochastic model and correction method for bacterial source tracking. Environmental Science and Technology 42:524-529.
Lindstrom, NM, DR Call, ML House, CM Moffitt, and KD Cain. In press. A quantitative enzyme-linked immunosorbent assay (ELISA) and filtration-based fluorescent antibody test as potential tools for screening Flavobacterium psychrophilum in broodstock. Journal of Aquatic Animal Health.
Lloyd, SJ, KR Snekvik, S St-Hilaire, SE LaPatra, KD Cain, and DR Call. 2008. Strawberry disease lesions in rainbow trout (Oncorhynchus mykiss) are closely associated with a Rickettsia-like organism. Disease of Aquatic Organisms 82:111-118. PMID: 19149374.
Meng, D, SL Broschat, and DR Call. 2008. A Java-based tool for the design of classification microarrays. BMC Bioinformatics 9:328. PMID: 18680597.
Shah, DH, KD Cain, GD Wiens, and DR Call. 2008. Challenges associated with heterologous expression of Flavobacterium psychrophilum proteins in Escherichia coli. Marine Biotechnology 10:719-730.
Stanton, JB, DP Knowles, DR Call, BA Mathison, and TV Baszler. In press. Limited transcriptional response of ovine microglia to prion accumulation. Biochemical and Biophysical Research Communications.
Whitworth, JH, N Fegan, J Keller, KS Gobius, JL Bono, DR Call, CJ Hovde, DD Hancock, and TE Besser. 2008. At an international scale, E. coli O157:H7 Stx bacteriophage insertion genotypes have a variable distribution between human and cattle hosts. Applied and Environmental Microbiology 74:7447-7450. PMID: 18849446.
Zhou, X, DH Shah, ME Konkel, and DR Call. 2008. Type III secretion system 1 genes in Vibrio parahaemolyticus are positively regulated by
ExsA and negatively regulated by ExsD. Molecular Microbiology 69:747-764. PMID: 18554322.
Zhou, X, ME Konkel, and DR Call. 2009. Type III secretion system 1 of Vibrio parahaemolyticus induces oncosis in both epithelial and monocytic cell lines. Microbiology 155:837-851. PMID: 19246755.
