College of Veterinary Medicine

Research in IPN

Raymond Quock, Ph.D.


Allen I. White Professor and Department Chair
Pharmaceutical Sciences, College of Pharmacy
Pharmacy site:
Phone: (509) 335-5956

Hyperbaric oxygen (HBO2) therapy has been approved by the FDA for a limited set of clinical indications, although there are clinical reports that HBO2 therapy appears to be effective in a broader range of conditions, including several examples of chronic pain (complex regional pain syndrome, fibromyalgia syndrome, migraine headache, rheumatoid arthritis, chronic osteomyelitis and other conditions).  Exposure to HBO2 causes a non-uniform increase in levels of NO metabolites (nitrate and nitrite) in various brain regions and spinal cord of rats indicating that HBO2 can stimulate production of NO.  Enhanced NO activity in the central nervous system may underlie some of the behavioral effects of HBO2.  We were the first to systemically investigate the pattern of analgesic response to single and multiple HBO2 treatments and to hypothesize a mechanism of analgesic effect.  HBO2-induced antinociception is sensitive to antagonism by inhibitors of NO production and opioid receptor antagonists, suggesting that the antinociception is mediated by NO and opioid mechanisms.  Repeated exposure to HBO2 produces an antinociceptive response of unusually long duration, possibly similar to the clinical reports of relief of chronic pain conditions by intermittent HBO2 treatments.  We are currently broadening our investigation of HBO2-induced antinociception to determine whether it is effective in suppression of neuropathic pain and whether gene changes might underlie its unusually long duration of action.

Nitrous oxide (N2O or more commonly known as laughing gas) is another medical gas that is notable for its anesthetic, analgesic, anxiolytic and euphoric properties.  We identified and localized in the rat brain the opioid receptor subtypes that mediate nitrous oxide antinociception, provided the first chemical evidence for N2O-induced neuronal release of endogenous opioid peptides in rats and have implicated a regulatory role for nitric oxide (NO) in the neuronal release of opioid peptides.  We have also reported that N2O produces significant relief of anxiety in different animal models of experimental anxiety.  This anxiolytic effect is independent of the antinociceptive effect of N2O and appears to be mediated by benzodiazepine sites on the g-aminobutyric acid (GABA) receptor and also involve NO.  This anxiolytic effect also appears to be mimicked by HBO2.

The present research into the mechanisms of pharmacological action of HBO2 and N2O utilizes a combination of stereotaxic neurosurgical, behavioral, pharmacological, neurochemical, microdialysis, pharmacogenetic and molecular biology approaches.

Biographical Information

Raymond M. Quock, Professor, obtained his B.S. degree in biology from the University of San Francisco in 1970 and his Ph.D. in pharmacology from the University of Washington in 1974. He spent one year as an instructor in pharmacology at the University of Washington (1974-75) and the next four years as an assistant professor of pharmacology at the University of Pacific School of Pharmacy in Stockton, California (1975-79).  Dr. Quock then worked at the Marquette University School of Dentistry in Milwaukee, Wisconsin (1979-89), moving through the ranks of assistant professor, associate professor and eventually to full professor. He also held adjunct positions at the Medical College of Wisconsin and the V.A. Medical Center.  He then left Milwaukee for the University of Illinois College of Medicine at Rockford (1989-98), where he was professor of pharmacology and also adjunct professor of pharmacology in anesthesiology at the University of Illinois College of Medicine in Chicago (1993-98).  Dr. Quock joined Washington State University as professor and chair of Pharmaceutical Sciences in the College of Pharmacy in January 1999.  He is a member of both the Neuroscience and Pharmaceutical Sciences graduate programs.  After returning to teaching and research in 2002. He was appointed to a second term as department chair in July 2007 and was named the Allen I. White Professor in August 2007.

Recent Publications

L.M. Zelinski, Y. Ohgami, E. Chung, D.Y. Shirachi and R.M. Quock.  A prolonged NO-dependent, opioid-mediated antinociceptive effect of hyperbaric oxygen in mice.  Journal of Pain 10:167-172 (2009).

M. Ishikawa, J. Moyer, J.A. Wolf, P. Mu, R.M. Quock, N.M. Davies, X.T. Hu, O.M. Schl├╝ter and Y. Dong.  Homeostatic synapse-driven membrane plasticity in nucleus accumbens neurons. Journal of Neuroscience 29:5820-5831 (2009)

Y. Ohgami, C.C. Zylstra, L.P. Quock, E. Chung, D.Y. Shirachi and R.M. Quock.  Nitric oxide in hyperbaric oxygen-induced acute antinociception in mice.  NeuroReport 20:1325-1329 (2009)

L.M. Zelinski, Y. Ohgami and R.M. Quock.  Exposure to nitrous oxide stimulates a nitric oxide-dependent neuronal release of β-endorphin in ventricular cisternally-perfused rats.  Brain Research 1300:37-40 (2009)

J.L. Cope, E. Chung, Y. Ohgami and R.M. Quock.  Antagonism of the antinociceptive effect of nitrous oxide by inhibition of enzyme activity or expression of nitric oxide synthase.  European Journal of Pharmacology 626:234-238 (2010)

E. Chung, L.M. Zielinski, Y. Ohgami, D.Y. Shirachi and R.M. Quock.  Hyperbaric oxygen treatment induces a two-phase antinociceptive response of unusually long duration in mice.  Journal of Pain (in press, 2010).  E-publication 23 Apr 2010. E-publication 23 Apr 2010. E-publication 23 Apr 2010.  

Last Edited: Apr 09, 2013 10:20 AM   

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