College of Veterinary Medicine

Research in IPN

Parijat Sengupta, Ph.D.

  Parijat Sengupta, Ph.D.
Research Assistant Professor
Office: Simasko Lab
Phone: 509-335-7920
Fax: 509-335-4650  

Research interests:

Molecular and Cellular Biophysics and High End Fluorescence Microscopy

My research endeavors focus on deciphering protein-protein interactions in living cells relevant to signal transduction. Currently I am working on two research projects. The first one aims to understand the role of purine type 2 receptor (P2R) in sleep regulation. Preliminary data from Dr. James M Kruegers lab suggest that P2R KO animals show different sleep phenotype. I am engaged in understanding this difference at the molecular level in co-culture of neurons and glial cells. The second project is on modeling sleep functional states at the cellular level. I am developing a novel brain cell-culture system for characterizing genetic influences on sleep-wake related state oscillations within the cultures. This system will be used to test the hypothesis of local and use-dependent sleep put forward by Dr. James M Krueger and his co-workers.
I am using molecular biology, electrophysiology, advanced fluorescence microscopy (confocal and two-photon microscopy, Forster resonance energy transfer) and fluorescence fluctuation techniques (FCS: Fluorescence correlation spectroscopy, ICS: image correlation spectroscopy and PCH: Photon counting histogram), for my research.
Biographical Information

Parijat Sengupta earned her BSc degree from Presidency College, Kolkata, India (1995) and MSc degree from Indian Institute of Technology, Kanpur, India (1997). She worked with Prof. Sudipta Maiti on her Ph.D. thesis at the Tata Institute of Fundamental Research, Mumbai, India and received her degree in physical chemistry and biophysics (2003). From 2004-05 she worked as a research assistant with Prof. Enrico Gratton in the Laboratory of Fluorescence Dynamics, University of Illinois at Urbana Champaign; from 2005-08 she was a research associate with Profs. Stuart McLaughlin and Suzanne Scarlata in the physiology and biophysics department of the Stony Brook University Medical Center. She joined WSU programs of neuroscience in IPN (formerly VCAPP) in November, 2008.

Selected Publications

Sensitive measurement of absolute two-photon absorption cross-sections. Parijat Sengupta, J. Balaji, S. Banerjee, R. Philip, G. Ravindra Kumar and S. Maiti, J. Chem. Phys., 112 (2000) 9201.

Probing diffusion and photochemical properties through localized photobleaching. J. Balaji, Parijat Sengupta, and S. Maiti, Multiphoton Microscopy in the Biomedical Sciences, Editors: Ammasi Periasamy, Peter T. C. So., Proceedings of SPIE, 4262 (2001) 329.

Determination of the absolute two-photon absorption cross section of tryptophan. Parijat Sengupta, J. Balaji, S. Mukherjee, R. Philip, G. Ravindra Kumar and S. Maiti, Multiphoton Microscopy in the Biomedical Sciences, Editors: Ammasi Periasamy, Peter T. C. So., Proceedings of SPIE, 4262 (2001) 336.

Measuring diffusion in cell membranes by fluorescence correlation spectroscopy. Parijat Sengupta, J. Balaji and S. Maiti, Methods, 27 (2002) 374. 

Aggregation of Alzheimers amyloid beta peptide studied by fluorescence correlation spectroscopy. Parijat Sengupta, K. Garai and S. Maiti, Proceedings of Sixth International Conference on Optoelectronics, Fiber Optics and Photonics, Mumbai, India, (2002) 51.

Localized optical probing of biomolecules: Fluorescence correlation spectroscopy and multi-photon microscopy. Parijat Sengupta and S. Maiti, Proc. Ind. Natl. Sci. Acad., (2003) 1.

Measuring size distribution in highly heterogeneous systems with fluorescence correlation spectroscopy. Parijat Sengupta, K. Garai, J. Balaji, N. Periasamy and S. Maiti, Biophys. J., 84 (2003) 1977.

The amyloid β peptide, Aβ1-40, is thermodynamically soluble at physiological concentrations. Parijat Sengupta, K. Garai, Yuan Shi, David J. E. Callaway and S. Maiti, Biochemistry, 42 (2003) 10506.

Fluctuation correlation spectroscopy with a laser scanning microscope: Exploiting the hidden time structure. Michelle A. Digman, Parijat Sengupta, Paul Wiseman, Claire M. Brown, Alan R. Horwitz and Enrico Gratton, Biophys. J., 88 (2005) L33.

Measuring fast dynamics in solution and cells with a laser scanning microscope.  Michelle A. Digman, Claire M. Brown, Parijat Sengupta, Paul Wiseman, Alan R. Horwitz and Enrico Gratton, Biophys. J., 89 (2005) 1317.

Selective destabilization of soluble amyloid β oligomers by divalent metal ions. K. Garai, Parijat Sengupta, B. Sahoo and S. Maiti, Biochem. Biophys. Res. Commun., 345 (2006) 210.

The membrane-permeable calmodulin inhibitors (e.g.W-7/W-13) bind to membranes, changing the electrostatic surface potential: Dual effect of W-13 on EGFR activation. Parijat Sengupta, Mara Jos Ruano, Francesc Tebar, Urszula Golebiewska, Irina Zaitseva, Carlos Enrich, Stuart McLaughlin and Antonio Villalobo, J. Biol. Chem. 282 (2007) 8474.

Signaling through the bradykinin receptor type 2 and Gαq follows a stoichometrically limited model. Finly Philip, Parijat Sengupta and Suzanne Scarlata, J. Biol. Chem. 282 (2007) 19203.

Quasi-homogeneous nucleation of amyloid beta yields numerical bounds for the critical radius, the surface tension and the free energy barrier for nucleus formation. K. Garai, B. Sahoo, Parijat Sengupta and S. Maiti, J. Chem. Phys. 128 (2008) 45102.

Caveolin-1 alters calcium signal duration through specific interaction with the Gαq family of G proteins. Parijat Sengupta, Finly Philip and Suzanne Scarlata, J. Cell Sci. 121 (2008) 1363.

EGFR Juxtamembrane Domain, Membranes and Calmodulin: Kinetics of Their Interaction. Parijat Sengupta, Eran Bosis, Esther Nachliel, Menachem Gutman, Steven O. Smith, Gyngyi Mihlyn, Irina Zaitseva and Stuart McLaughlin, Biophys. J. (2009) in press.

Last Edited: Apr 09, 2013 10:22 AM   

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