Research Assistant Professor
Office: Simasko Lab
Molecular and Cellular Biophysics and High
End Fluorescence Microscopy
My research endeavors focus on deciphering protein-protein
interactions in living cells relevant to signal transduction. Currently
I am working on two research projects. The first one aims to understand
the role of purine type 2 receptor (P2R) in sleep
regulation. Preliminary data from Dr. James M Kruegers lab suggest that
P2R KO animals show different sleep phenotype. I am engaged in
understanding this difference at the molecular level in co-culture of
neurons and glial cells. The second project is on modeling sleep
functional states at the cellular level. I am developing a novel brain
cell-culture system for characterizing genetic influences on sleep-wake
related state oscillations within the cultures. This system will be used
to test the hypothesis of local and use-dependent sleep put forward by
Dr. James M Krueger and his co-workers.
I am using molecular biology, electrophysiology, advanced fluorescence
microscopy (confocal and two-photon microscopy, Forster resonance energy
transfer) and fluorescence fluctuation techniques (FCS: Fluorescence
correlation spectroscopy, ICS: image correlation spectroscopy and PCH:
Photon counting histogram), for my research.
Parijat Sengupta earned her BSc degree from Presidency
College, Kolkata, India (1995) and MSc degree from Indian Institute of
Technology, Kanpur, India (1997). She worked with Prof. Sudipta Maiti on
her Ph.D. thesis at the Tata Institute of Fundamental Research, Mumbai,
India and received her degree in physical chemistry and biophysics
(2003). From 2004-05 she worked as a research assistant with Prof.
Enrico Gratton in the Laboratory of Fluorescence Dynamics, University of
Illinois at Urbana Champaign; from 2005-08 she was a research associate
with Profs. Stuart McLaughlin and Suzanne Scarlata in the physiology and
biophysics department of the Stony Brook University Medical Center. She
joined WSU programs of neuroscience in IPN (formerly VCAPP) in November,
Sensitive measurement of absolute two-photon absorption cross-sections.
, J. Balaji, S. Banerjee, R. Philip, G.
Ravindra Kumar and S. Maiti, J. Chem. Phys., 112 (2000) 9201.
Probing diffusion and photochemical properties through localized
photobleaching. J. Balaji, Parijat Sengupta
, and S.
Maiti, Multiphoton Microscopy in the Biomedical Sciences, Editors:
Ammasi Periasamy, Peter T. C. So., Proceedings of SPIE, 4262 (2001) 329.
Determination of the absolute two-photon absorption cross section of
tryptophan. Parijat Sengupta
, J. Balaji, S. Mukherjee,
R. Philip, G. Ravindra Kumar and S. Maiti, Multiphoton Microscopy in the
Biomedical Sciences, Editors: Ammasi Periasamy, Peter T. C. So.,
Proceedings of SPIE, 4262 (2001) 336.
Measuring diffusion in cell membranes by fluorescence correlation
spectroscopy. Parijat Sengupta
, J. Balaji and S. Maiti,
Methods, 27 (2002) 374.
Aggregation of Alzheimers amyloid beta peptide studied by fluorescence
correlation spectroscopy. Parijat Sengupta
, K. Garai
and S. Maiti, Proceedings of Sixth International Conference on
Optoelectronics, Fiber Optics and Photonics, Mumbai, India, (2002) 51.
Localized optical probing of biomolecules: Fluorescence correlation
spectroscopy and multi-photon microscopy. Parijat Sengupta
and S. Maiti, Proc. Ind. Natl. Sci. Acad., (2003) 1.
Measuring size distribution in highly heterogeneous systems with
fluorescence correlation spectroscopy. Parijat Sengupta
K. Garai, J. Balaji, N. Periasamy and S. Maiti, Biophys. J., 84 (2003)
The amyloid Î² peptide, AÎ²1-40, is thermodynamically soluble at
physiological concentrations. Parijat Sengupta
Garai, Yuan Shi, David J. E. Callaway and S. Maiti, Biochemistry, 42
Fluctuation correlation spectroscopy with a laser scanning microscope:
Exploiting the hidden time structure. Michelle A. Digman,
, Paul Wiseman, Claire M. Brown, Alan R.
Horwitz and Enrico Gratton, Biophys. J., 88 (2005) L33.
Measuring fast dynamics in solution and cells with a laser scanning
microscope. Michelle A. Digman, Claire M. Brown, Parijat
, Paul Wiseman, Alan R. Horwitz and Enrico Gratton,
Biophys. J., 89 (2005) 1317.
Selective destabilization of soluble amyloid Î² oligomers by divalent
metal ions. K. Garai, Parijat Sengupta
, B. Sahoo and S.
Maiti, Biochem. Biophys. Res. Commun., 345 (2006) 210.
The membrane-permeable calmodulin inhibitors (e.g.W-7/W-13) bind to
membranes, changing the electrostatic surface potential: Dual effect of
W-13 on EGFR activation. Parijat Sengupta
, Mara Jos
Ruano, Francesc Tebar, Urszula Golebiewska, Irina Zaitseva, Carlos
Enrich, Stuart McLaughlin and Antonio Villalobo, J. Biol. Chem. 282
Signaling through the bradykinin receptor type 2 and GÎ±q follows a
stoichometrically limited model. Finly Philip, Parijat Sengupta
and Suzanne Scarlata, J. Biol. Chem. 282 (2007) 19203.
Quasi-homogeneous nucleation of amyloid beta yields numerical bounds for
the critical radius, the surface tension and the free energy barrier for
nucleus formation. K. Garai, B. Sahoo, Parijat Sengupta
and S. Maiti, J. Chem. Phys. 128 (2008) 45102.
Caveolin-1 alters calcium signal duration through specific interaction
with the GÎ±q family of G proteins. Parijat Sengupta
Finly Philip and Suzanne Scarlata, J. Cell Sci. 121 (2008) 1363.
EGFR Juxtamembrane Domain, Membranes and Calmodulin: Kinetics of Their
Interaction. Parijat Sengupta
, Eran Bosis, Esther
Nachliel, Menachem Gutman, Steven O. Smith, Gyngyi Mihlyn, Irina
Zaitseva and Stuart McLaughlin, Biophys. J. (2009) in press.