Pathogen detection and genotyping MicroArray Laboratory
DNA microarrays can serve as "end-point" detectors when coupled to polymerase
chain reaction (PCR). This assay design introduces considerable flexibility
because multiple polymorphisms can be distinguished for conserved genes (e.g.,
16S rDNA) and more complex multiplex reactions can be interrogated without
reliance upon fragment size discrimination using electrophoresis.
Projects
Salmonid pathogen detection
Fish pathogens are particularly difficult to screen and identify because,
in general, these are fastidious organisms requiring a heterogeneous set of
assays for conventional detection (ELISA, FA, selective plating, cell
culture). We reasoned that a 16S rDNA-based detector could be very useful
for both aquaculture diagnostics and for studying the ecology and these
organisms.
Presentations
- Northwest Science, Forks WA, March 2003
- American Society for Microbiology, May
2003,
Abstract
- American Fisheries Society, Boise, ID, 16 June 2003
- AFS-FHS Annual meeting, Seattle, WA, July 2003
Publications
-
Call, DR, MK Borucki and FJ Loge. 2003. Detection of bacterial
pathogens in environmental samples using DNA microarrays. Journal of
Microbiological Methods 53:235-243.
Abstract
-
Warsen, AE, DR Stanek, FJ Loge, and DR Call. In prep. Simultaneous
detection of multiple salmonid pathogens using DNA microarrays.
-
González, SF, MJ Krug, ME Nielsen, Y Santos, and DR
Call. In review. Simultaneous detection of marine fish pathogens using a
multiplexed PCR and DNA microarrays.
-
Lane, S., J. Evermann, F. Loge and D.R. Call. In
review. Amplicon secondary structure, labeling strategies, and
implications for microarray assays.
Funding
-
WSU and UI Center for Reproductive Biology (Pullman,
WA), Salmon Restoration Program, U.S. Fish and Wildlife Service. Assay
development and application.
-
Pacific Northwest Aquaculture Initiative (Pullman,
WA), Validation of 16S rDNA microarray using Renibacterium as a model
system.
-
Washington Water Research Center (Pullman, WA),
Testing 16S rDNA microarray with hatchery in-flow and out-flow waters.
-
Agricultural Animal Health Program,
Pullman, WA, Funding technical support and instrumentation.
Last Edited: Dec 26, 2007 3:56 PM
