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An Alphabetized List of PAgesAn Alphabetized List of PAges
  Genomics    

Directors: Kelly A. Brayton and Douglas R. Call

Goals:

  • Encourage interdisciplinary collaborations and graduate and post-graduate research opportunities.
  • Examine genetic diversity as it relates to differential survivorship and virulence.
  • Identify mechanisms responsible for generating genetic diversity.
  • Develop new tools for diagnostics and strategies for pathogen control and intervention

.Links to Projects:

Genome sequencing - Kelly A. Brayton
Anaplasma marginale
Babesia bovis

Microarrays - Douglas R. Call
Detectors
ComparativeGenomics  
Expression Arrays

Publications related to this program:

  • Borucki, M. K., M. J. Krug, W. T. Muraoka, and D. R. Call (2003). Discrimination among Listeria monocytogenes isolates using a mixed genome DNA microarray. Veterinary Microbiology 92:351-362.

  • Brayton, K. A., D. P. Knowles, T. C. McGuire, and G. H. Palmer (2001). Efficient use of a small genome to generate antigenic diversity in tick-borne ehrlichial pathogens.  Proceedings of the National Academy of Sciences 98:4130-4135.
  • Call, D.R., M.K. Borucki, and T.E. Besser. (2003). Mixed-genome microarrays reveal multiple serotype and lineage-specific differences for Listeria monocytogenes. Journal of Clinical Microbiology 41:632-639.

  • Call, D.R., M.K. Borucki and F.J. Loge. (2003). Detection of bacterial pathogens in environmental samples using DNA microarrays. Journal of Microbiological Methods 53:235-243.
  • Call, D.R. 2001. DNA microarrays – their mode of action and possible applications in molecular diagnostics. Veterinary Sciences Tomorrow, Issue 3, August 2001.
  • Call, D.R., F. J. Brockman, and D. P. Chandler (2001). Detecting and genotyping Escherichia coli O157:H7 using multiplexed PCR and nucleic acid microarrays. International Journal of Food Microbiology 67:71-80.
  • Call, D.R., D.P. Chandler, and F.J. Brockman. (2001). Fabrication of DNA microarrays using unmodified oligomer probes. BioTechniques 30:368-379
  • Chandler, D.P., Brown, J., Call, D.R., Grate, J.W., Holman, D.A., Olson, L., Stottlmyre, M.S., and C.J. Bruckner-Lea. (2001).  Continuous, automated immunomagnetic separation and microarray detection of E. coli O157:H7 from poultry rinsate. Intl. J. Food Microbiology 70:143-154.
  • Kingsley, M.T., Straub T.M., Call D.R., Wunschel S.C., Daly D.S. and D.P. Chandler. (in press). Fingerprinting closely related Xanthamonas pathovars with random nonamer oligonucleotide microarrays. Applied and Environmental Microbiology
  • Rurangirwa, F. R., K. A. Brayton, T. C. McGuire, D. P. Knowles, and G. H. Palmer (2002). Conservation of the unique rickettsial rRNA gene arrangement in Anaplasma International Journal of Systematic and Evolutionary Microbiology. 52: 1405-1409.

 

Program accomplishments:

  • Anaplasma sequence
  • Developed mixed genome microarray concept with Listeria monocytogenes as a model system. This concept is being applied to other problems in molecular epidemiology, food safety, comparative genomics and water quality assessment.
  • Developing arrays suitable for simultaneous detection of multiple salmonid pathogens.
Example of genomic hybridizations for two strains of L. monocytogenes (serotypes 1/2a, top and 4b, bottom). Only a portion of the full array is shown. Spots clearly visible indicate presence of target. Missing spots are attributable to absent or highly diverged sequence (e.g., column 1, row 4).

This Program receives support from the following sources:

National Institutes of Health (NIH)

USDA-NRIGCP

USDA-ARS ADRU Microbial Genomics Initiative

Morris Animal Foundation

Washington State University:

  • Agricultural Animal Health Program

  • Salmon Restoration Program (USFWS)

  • Center for Reproductive Biology

  • NW Aquaculture Initiative (USDA)

  • Washington Water Research Center

 
Revised December 06, 2005     |     Printer Friendly Version

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