CD83 HB15
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 1 glycoprotein
Spleen
Brain
Polymorphonuclear cell
Langerhans Cell
Interdigitating reticulum cell
Dendritic Cell
Lymphoblastoid cell
T Cell
B Cell
Hodgkin lymphoma
40 / 40
45 / 45

Expression
CD83 is expressed on non-follicular, circulating, and interdigitating dendritic cells within lymphoid tissues, Langerhans cells, in vitro generated thymic dendritic cells and polymorphononuclear cells.  Expression reacts with some EBV-transformed lymphoblastoid cell lines, Hodgkin cells and with some germinal B cells in vivo.  CD83 is used as a marker for a subset of mature dendritic cells.  In the mouse, CD83 is expressed in the brain and spleen but it is not specific for dendritic cells in human. 

Structure
MOLECULAR FAMILY NAME: Belongs to the immunoglobulin supergene family.

CD83 is a single-pass type-1 glycoprotein.  It contains an extracellular domain which contains an Ig-like V-type domain and is heavily glycosylated, a transmembrane and a 39 aa short C-terminal cytoplasmic domain.  The gene for CD83 has an unusual structure, with respect to most other IgSF molecules, in that the intron/exon boundaries encompassing the IgSF domain are of phase 0 and phase 1 rather than both being of phase 1.  There is also an intron within the coding sequence for the IgSF domain.

MOLECULAR MASS
Cell Type Unreduced Reduced
cDNA-transfected K562 cells 43 kDa

POST-TRANSCRIPTIONAL MODIFICATION:

Alternative splicing yields 3 different isoforms, CD83a, b and c.  Only one isoform, CD83c, has thus far been found to be a functional soluble isoform detectable in serum.

POST-TRANSLATIONAL MODIFICATION

CD83 is heavily glycosylated.

Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH CD83: No information.



Function
CD83 is a dendritic cell marker.  CD83 may play a role in antigen presentation and/or lymphocyte activation and regulates immune reponse and may be involved in activating immune effector cells.  Knockout mice have arrested development of CD4+ single-positive cells.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD83 IN INTACT ANIMAL 

CD83 levels are increased in serum of leukemia patients.

Comments
MOLECULAR INTERACTIONS -
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD83: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD83: No information.

ADDITIONAL INSIGHTS

CD83 is currently one of the best markers for human mature dendritic cells.

Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 9308Q01151
Antibodies
HB15A   View Reactivity
HB15E   View Reactivity

Revised June 25, 2008


Contact us: Webmaster |  509-335-9515 | Accessibility | Copyright | Policies
College of Veterinary Medicine Washington State University, Pullman, WA, 99164-7010 USA
Copyright 1995-2003 Washington State University