CD111 PRR1 (poliovirus receptor-related 1 protein), PVRL1, Nectin 1, Hve C1
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 1 glycoprotein
Stem Cell
Trachea
Hematopoietic Cell
Myeloid Cell
Endothelial Cell
Fibroblast
Epithelial Cell
Kidney
Brain
Placenta
Prostate
Pancreas
Skin
Lung
Liver
Megakaryocyte
Thyroid
Myelomonocytic cell
Plasma Cell
Neuronal Cell
Erythroid Cell
Spinal cord
75 / 75

Expression
CD111, an intracellular adhesion molecule, is expressed on multiple cell types in the brain, spinal cord, trachea, prostate, placenta, skin, kidney, lung, pancreas, thyroid, and liver.  CD111 is expressed on cell lines of different lineages, including myeloid cells, and neuronal cells.  It is found in the plasma membrane, intra-cellularly, and in vesicle-like structures.  CD111 is expressed on CD33+, CD14+ and CD41+ cells.  It is also found on CD34 + stem cells, myelomonocytic cells, precursors of red cells and platelets and outside the hematopoietic lineage in erythroid lineages, fibroblastic cells, neuronal cells epithelial cells, and endothelial cells.  CD111 is a component of the adherens junction and is 1 of several receptors for herpes simplex viruses types 1 and 2.


Structure
MOLECULAR FAMILY NAME: Belongs to the immunoglobulin gene family.

CD111 is a single-pass type-1 glycoprotein.  It contains an extracellular domain which contains 2 Ig-like C2-type domains and 1 Ig-like V-type domain and has 8 potential N-glycosylation sites, a transmembrane domain and a cytoplasmic domain.  CD111 was originally identified as a transmembrane molecule related to the CD155 molecule and named poliovirus receptor related 1 (PRR1).  CD111 belongs to a new family of immunoglobulin-like molecules that includes 4 members, CD111 (nectin-1), CD112 (nectin-2), CD113 (nectin-3) and CD155 (nectin-like 5).  CD111 shows approximately 30% homology with CD112 and CD113.  The gene encodes at least 2 different transmembrane isoforms sharing identical ectodomains but different transmembrane and cytosolic regions.  The 2 corresponding transcripts of 5.9 kb and 3.5 kb are detectable in several tissues.  Apparent Mr of the long form of CD111 is 75 kDa.

MOLECULAR MASS1
Cell Type Unreduced Reduced
TF1 cell line 75kDa

POST-TRANSCRIPTIONAL MODIFICATION

Alternative splicing yields 3 different isoforms, 1554 bp and 1377 bp long, that splices at position 1003 in the ectodomain.  Transmembrane and cytoplasmic sequences are unrelated.  The long isoform is the predominant expressed form (518 aa) and the shorter isoform (459 aa) is also observed.

POST-TRANSLATIONAL MODIFICATION

CD111 has 8 potential N-glycosylation sites.


Ligands
The extracellular region of CD111 binds the related nectins, CD112, CD113, nectin-4 and CD155 (poliovirus ligand).  It can interact with nectin-1 on the same or on other cells (i.e. cis- and trans-homo-interaction).  CD111 is a receptor for the α herpes viruses HSV-1 and 2 and pseudorabies virus PRV. 

LIGANDS AND MOLECULES ASSOCIATED WITH CD111
Molecule Comment
CD111 ligand
nectin 3 CD111 ligand
afadin CD111 associated molecule
gD gD binds to the V domain of CD111


Function
CD111 is an adhesion molecule involved in the formation of adherens junctions between epithelial cells, cell physiology and trans-interacts with nectin 3.  An isoform of CD111 localizes specifically at the adherens junctions via its cytoplasmic interaction with the scaffold F-actin cytokeleton binding protein through afadin.  The extracellular portion interacts with the other nectin family members, CD112, CD113 and CD155.  This interaction is mediated by a sequence located to the C terminal end of CD111, A/ExYV, and the PDZ domain of afadin.  This sequence is also found in CD112 and nectin 3 and represents a specific consensus in the family which is also named the nectin family.  The function of CD111 on hematopietic cells is not known.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD111 IN INTACT ANIMAL

CD111 is associated with cleft lip/palate ectodermal dysplasia syndrome, ectodermal dysplasia, and Margarita Island type Zlotogora-Ogur syndrome.  CD111 is a receptor for the α herpes viruses HSV-1 and 2 and pseudorabies.  The soluble chimeric CD111-Fc fusin protein and antibody against CD111 blocks virus entry in vitro.  The R1.302 monoclonal antibody recognizes the V-type domain of the molecule and blocks virus entry and cell to cell spreading.  CD111 may function in homophilic and intracellular adhesion and as a coreceptor for HSV-1 and HSV-2 during infection of epithelial cells on mucosal surfaces.  CD111 is a pan-a herpesvirus entry receptor, also named HveC / HlgR, as it mediates entry of many HSV1 and HSV2 strains, bovine herpesvirus 1 (BHV-1) and porcine pseudorabies virus (PRV).  The V domain of CD111 interacts with the glycoprotein D of the virus and is sufficient to confer susceptibility to a herpesvirus infection.  CD111 is involved in cell to cell spreading of the virus.  Murine CD111 also acts as a HSV, BHV-2 and PrV receptor and binds to the to the viral glycoprotein D.  Glycoprotein D interaction is weaker than with human CD111.



Comments
MOLECULAR INTERACTIONS -
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD111: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD111: No information.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 5818Q15223
Antibodies

Revised June 25, 2008


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