CD134 TNFRSF4 (tumor necrosis factor receptor superfamily, member 4), OX40, CD252
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Lineage Restricted Molecule
Type 1 glycoprotein
T Cell
B Lymphocyte
Lymphoblast
B Cell
Hematopoietic Cell
Fibroblast
Endothelial Cell
T Lymphocyte
50 / 50

Expression
CD134 is specifically expressed on activated and regulatory T cells with the maximum expression 24 hours after stimulation.  In the rat, CD134 is only found on activated CD4+ T cells with 32,000 sites per cell and in human it is described  predominantly on CD4+ cells.  In mouse, CD134 is expressed on both activated CD4+ and CD4+CD8a+ cells.  CD134 is expressed on fibroblasts, hematopoietic precursors and on activated T and B lymphocytes. It is also expressed on HTLV-1 transformed and activated B lymphoblastoid and monocytic cell lines and on vascular endothelial cells. 

Structure
MOLECULAR FAMILY NAME: Belongs to the tumor necrosis factor receptor family.

CD134 is a single-chain type-1 glycoprotein.  It contains a 277 aa extracellular domain which contains 3 complete Cys-rich repeats and 2 N-glycosylation sites, a transmembrane-proximal hinge domain which contains possible sites for O-linked glycosylation and cytoplasmic domain.

MOLECULAR MASS
Cell Type Unreduced Reduced
51 kDa-55 kDa 47 kDa-51 kDa

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION: No information.


Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH CD134

CD134 binds to OX40L and there is no evidence for another ligand. The affinity of monomeric CD134 for  CD252 (OX40L) is 190 nM and the affinity of trimeric CD252 for CD134 on the surface of activated T cells is 0.2 nM.  here are 3 CD134 receptors that bind 1 CD252 trimer.



Function
CD134 has been shown to activate NF-κB through its interaction with adaptor proteins TRAF2, TRAF3 and TRAF5.  Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors, BLC2, and thus suppresses apoptosis, as well as in T cell-dependent B cell proliferation and differentiation.  Blocking the CD252/CD134 interaction reduced IgG production, suggesting a role in differentiation into plasma cells.  CD252 is a costimulatory molecule of T cells, binding to CD252 (OX ligand) on antigen presenting cells including B cells.  Ligation of the receptor is inhibitory for apoptosis, and the receptor also appears to be involved in B cell response to T dependent antigens which co-stimulates proliferation, activation, cell adhesion, and differentiation.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD134 IN INTACT ANIMAL

CD134 is upregulated at sites of inflammation in multiple sclerosis and in psoriatic lesions.


Comments
MOLECULAR INTERACTIONS-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD134: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD134: No information.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 7293P43489
MouseP43488U12763
MouseP47741Z21674
RatS12783P15725X17037
Antibodies
ACT35   View Reactivity

Revised June 25, 2008


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