CD140b PDGFRβ (platelet-derived growth factor receptor β polypeptide)
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 1 glycoprotein
Endothelial Cell
Stromal Cell
Mesenchymal Cell
180 / 180

Expression
CD140β is expressed on endothelial cells, subsets of stromal cells and on mesenchymal cells.

Structure
MOLECULAR FAMILY NAME:  Belongs to the tyrosine kinase receptor family.

CD140β is a single-pass type-1 glycoprotein.  It contains a 499 aa extracellular domain which contains 5 Ig-like C2-type domains and 11 potential N-linked glycosylation sites, a 25 aa transmembrane and a 550 aa intracellular cytoplasmic domain which contains a protein kinase domain which is split in two by an insert domain and 2 ATP-binding sites.  CD140β binding induces either homodimerization or heterdimerization with the CD140α molecule.

MOLECULAR MASS
Cell Type Unreduced Reduced
180 kDa

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION: No information.


Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH CD140β

CD140β binds platelet derived-growth factors.

Function
CD140β encodes a cell surface tyrosine receptor for members of the platelet-derived growth factor family.  These growth factor are mitogens for cell of mesenchymal origin.  The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer composed of both platelet-derived growth receptor α and β polypeptides.  CD140β mediated PI3K signaling is essential for cell migration and chemotaxis.  CD140β knockout mice have defective smooth muscle cell production with the most affected cell types being vascular smooth muscle and pericytes.  Studies in knockout mice, where homozygosity is lethal, indicate that the b form of the CD140β is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.  The vascular endothelium of the brain, heart, kidney, skin and the eye rely on CD140β signal transduction and it is probably involved in proliferation of these vascular endothelia.  CD140β plays an important role in would healing as it mediates chemotaxis/motility of fibrobasts.

BIOCHEMICAL ACTIVITY

The major biochemical difference between CD140α and CD140β is the preferential binding of RasGAP to CD140β but not to CD140α and Crk association  with CD140α but not CD140β.

DISEASE RELEVANCE AND FUNCTION OF CD140β IN INTACT ANIMAL

Defects in CD140β are found in a form of chronic myelomonocytic leukemia (CMML) and in acute myelogenous leukemia with eosinophilia.  CD140β is a promising therapeutic target in the treatment of several tumors particularly those tumors where autodrine CD140β stimulation is important.  The E5 protein of bovine papilloma virus binds the transmembrane of CD140β and induces dimerization and activation of the receptor.


Comments
MOLECULAR INTERACTIONS-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD140β: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD140β: No information.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 5159P09619
Antibodies

Revised June 25, 2008


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