CD147 BSG (basign OK blood group), EMMPRIN(extracellular matrix metalloproteinase inducer), M6
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 1 glycoprotein
Nonhematopoietic Cell
Leukocyte
Lymphocyte
Epithelial Cell
Hematopoietic Cell
Blood Cell
Platelet
Monocyte
T Cell
Cancer cell
Endothelium
Brain
Tumor Cell
Thymocyte
28 / 28
54 / 54

Expression
CD147 is expressed broadly on both hemopoietic and non-hemopoietic cells.  CD147 is expressed on leukocytes, red blood cells, platelets and endothelium cells.  Expression is strong on thymocytes and activated peripheral T cells but is less strong on resting peripheral T cells.  CD147 is expressed weakly on resting leukocytes but is strongly upregulated on activated lymphocytes and monocytes.  CD147 is expressed in vascular endotheliums in non-neoplastic regions of the brain and in tumor cells but not in blood vessels in malignant gliomas.  CD147 is expressed on various epithelial cells with some differences between species such as the rat, mouse and chicken.  Expression is strong on cancer cells in small lung cancer and hepatocellular carcinoma.

Structure
MOLECULAR FAMILY NAME: Belongs to the immunoglobulin gene superfamily.

CD147 is a single-chain type-1 269 aa glycoprotein.  It is contains a 21 aa N-terminal signal peptide, an 185 aa extracellular region which contains a C2-type domain and a V-type domain, a 21 aa putative transmembrane domain which contains a leucine-zipper and a charged glutamic acid residue, suggesting it is involved in protein-protein interactions and is highly conserved between the human, rat, mouse and chicken and a 40 aa cytoplasmic domain.  Within the transmembrane domain, the charged Glu is noteworthy because charged residues are rarely found in transmembrane segments of proteins with single membrane spanning segments, except in proteins which associate with other polypeptides in the membrane.  The transmembrane sequence of CD147 is absolutely conserved between human and chicken.  In the mouse, cDNA clones encoding different N-termini have been found.  This heterogeneity may be due to alternative splicing.  The extent of CD147 glycosylation is tissue-specific and is responsible for a variation in the apparent molecular mass from 28 kDa to 54 kDa. 

MOLECULAR MASS
Cell Type Unreduced Reduced Comment
HPB-ALL 50-60 kDa 55-65 kDa

POST-TRANSCRIPTIONAL MODIFICATION

Alternative splicing yields 2 different isoforms. 

POST-TRANSLATIONAL MODIFICATION

CD147 is N-glycosylated.  N-linked glycans are responsible for approximately 50% of the mass.

Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH CD147:

Adhesions and coimmunoprecipitation suggests CD147 binds to intregrins.


Function
CD147 is a potential cell adhesion molecule and is involved in the regulation of T cell function.  In vitro studies suggest CD147 is involved in adhesion, because CD147 antibodies inhibit homotypic aggregation and CD147 is coimmunoprecipitated with integrins.  There is a growing indication that there is a multipotent involvement of CD147 as a cell adhesion molecule or regulator of cell adhesion in immune cell function and migration.  Soluble CD147 protein stimulates collagen production, suggesting a regulatory activity on metalloproteinses.  CD147 stimulates matrix metalloproteinase synthesis in fibroblasts.  CD147, an extracellular matrix metalloproteinase inducer (EMMPRIN) on tumor cells is thought to bind an unknown ligand on fibroblasts, which stimulates their production of collagenase and other extracellular matrix metalloproteinases, thus enhancing tumor cell invasion and metastasis.  Soluble recombinant forms bind to various cells including endothelial cells and fibroblasts.  Certain mAbs inhibit aggregation of the breast cancer cell line MCF-7, as well as MCF-7 cell adhesion to type 4 collagen, laminin and fibronectin.  A mAb to the chicken orthologue reduces retina cell aggregation.  CD147 was 1st described as a tumor cell derived soluble factor or tumor cell derived collagenase stimulatory factor (TCSF) that stimulates expression of collagenases by fibroblasts.  CD147 knockout mice are infertile and suggest a variety of additional functions showing differences in memory and learning, odor perception and immunological function and their lymphocytes show an increased mitogenic response upon mixed lymphocyte reaction.  An adhesion role for chicken CD147 has been proposed, since the 5A11 mAb inhibits neural extensions of retinal glial cells and reduces retinal cell reaggregation in vitro.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD147 IN INTACT ANIMAL

CD147 plays a role in spermatogenesis, embrryo implantnation, neural network formation and tumor progression. 

Comments
MOLECULAR INTERACTIONS -
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD147: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD147: No information.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
ChickenS10147P17790X52751
HumanEntrezgene 682P35613
MouseJX0107P18572D00611
RatA45444P26453X54640
Antibodies

Revised June 25, 2008


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