|CD148||PTPRJ (protein tyrosine phosphatase, receptor type J), DEP-1 (high density-enhanced PTP 1), HPTP-η|
|Molecule Type||Antigen Expression||Molecular Weight|
Min / Max
|Non-lineage Restricted Molecule|
Type 1 glycoprotein
|200 / 200|
250 / 250
|CD148 is expressed among hematopoietic cells. Expression is strong on monocytes, granulocytes (mainly at sites of inflammation) and dendritic cells. Resting T cells express low levels but expression is upregulated following T-cell activation and CD45R0-psoitive express higher levels than naive cells. Memory T-cells show higher levels of expression. Platelets, fibroblasts, nerve cells, and Kupffer cells are also expressed. It is a widely distributed antigen.|
|MOLECULAR FAMILY NAME: Belongs to the protein tyrosine phosphatase family.|
CD148 is a single-chain, type-1 1337 aa glycoprotein. It is contains a 970 aa extracellular domain which contains 10 fibronectin III motifs, 34 potential N-linked glycosylation sites as well as O-glycosylation sites, a 25 aa transmembrane domain and a 342 aa intracellular cytoplasmic C-terminal domain which contains 1 protein tyrosine phosphatase domain.
Modification is unknown but possible.
There are 34 potential N-glycosylation sites. CD148 is O-glycosylated, sialydated, and is treated with endoglycosidase F. O-glycanase and neuraminidase reduces the molecular weight but phosphorylation is unknown.
|LIGANDS AND MOLECULES ASSOCIATED WITH CD148|
A natural ligand is unknown.
|CD148, a member of the protein tyrosine phosphatase (PTP) family, is known as a signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle and oncogenic transformation. CD148 is present in all hematopietic lineages and was shown to negatively regulate T cell receptor signaling possibly through interfering with phosophorylation. The levels of expression of CD148 increase on contact between epithelial cell lines suggesting a role in contact inhibition of cell growth. The role of CD148 in the immune system is unclear but engagement with the antibody leads to cytokine secretion. CD148 may have a role in arresting cell growth at confluence.|
CD148 is a tyrosine phosphatase RPTPase, type 3.
DISEASE RELEVANCE AND FUNCTION OF CD148 IN INTACT ANIMAL
Defects in CD148 are found in cancers of the colon, lung and breast.
|MOLECULAR INTERACTIONS -|
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD148: No information.
CD148 contains tyrosine phosphorylated proteins.
ENZYMES WHICH MODIFY CD148: No information.
Strong upregulation in fibroblast cultures grown to high density, could suggest that CD148 HPTP-eta/Dep-1 is involved in contact inhibition of cell growth.
Database accession numbers
Revised June 25, 2008