CD160 BY55, NK1, NK28
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Lineage Restricted Molecule
GPI anchor
T Cell
NK Cell
IEL
CTL
Small intestine
Spleen
Peripheral blood
Lymphocyte
27 / 27
80 / 80

Expression
CD160 is expressed at the cell surface as a disulfide-linked multimer.  Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity.  RNA blot analysis has revealed the presence of CD160 mRNAs of 1.5 kb and 1.6 kb in subsets of circulating NK and T cells and cells in the spleen and small intestine.  CD160 is expressed by CD56 dim CD16+ NK cells.   In blood, expression is mainly restricted to gd T cells and ab + CD8 bright CD95+CD56+CD28-CD27- T cells.   CD160 is expressed on all intestinal intraepithelial lymphocytes.  Receptor expression of CD160 is downregulated following T and NK cell activation.  CD160 is expressed on CTLs and IELs. 


Structure
MOLECULAR FAMILY NAME: Belongs to the immunoglobulin gene superfamily.

CD160 is a type-2 cysteine-rich GPI-anchored 181 aa glycoprotein  It contains an 181 aa extracellular domain which contains a Ig-like V-type domain which has 6 cysteines in the mature polypeptide and 2 N-linked glycosylation sites.  The Ig-like domain is weakly homologous to MHC binding receptors referred to as killer inhibitary receptors (KIR) and to the CD158d (KIR2DLA4) molecule.   CD160 is expressed at the cell surface as a tightly disulfide-linked multimer.    

MOLECULAR MASS
Cell Type Unreduced Reduced Comment
NK cell line YT 80 kDa 27 kDa CD160 is expressed at the cell surface as disulfide-linked multimer

CD160 is a 27 kDa glycoprotein which was initially identified with the mAb BY55.

POST-TRANSCRIPTIONAL MODICIATION: No information.

POST-TRANSLATIONAL MODIFICATION

CD160 has 2 N-linked glycosylations.


Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH CD160

CD 160 binds HLA-C molecules and displays broad specificity binding to classical and nonclassical MHC class I molecules.

Function
Cross-linking CD160 with certain mAbs or MHC class I provides a costimulatory signal to CD3-activated CD8+ T lymphocytes, enhancing their proliferation. After its engagement of an HLA-C molecule on a target cell, CD160 triggers cell cytotoxicity and the release of IFN-γ, TNF-α and IL-6 by NK cells.  Cell surface expression of CD160 is downregulated by cytokine-induced NK cell activation.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD160 IN INTACT ANIMAL

CD160 expression is altered in patients with paroxysmal nocturnal hemoglobinuria.


Comments
MOLECULAR INTERACTIONS -
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD160: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD160: No information.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene11126O95971
MouseO95971AF060982
Antibodies

Revised June 25, 2008


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