| 114/A10 |
| Molecule Type | Antigen Expression | Molecular Weight Min / Max |
| Non-lineage Restricted Molecule Type 1 glycoprotein | Hematopoietic Cell, Mouse Myeloid Cell Progenitor Cell Erythroid Cell | 95 / 305 |
Expression | ||||||||||||||
| The 114/A10 antigen is expressed on mouse hematopoietic cells and cell lines that are responsive to IL-3, including primary erythroid, myeloid and multi-potent progenitors. | ||||||||||||||
Structure | ||||||||||||||
| 114/A10 is a single chain type1 glycoprotein. The extracellular N-terminus is comprised of 8 highly conserved Ser/Thr-rich repeats of about 27 residues each, followed by 3 complete epidermal growth factor (EGF)-domains and 1 truncated EGF do-ain. Between EGF-domains 1 and 3 there is a region of 120 residues with similarity to the recently defined SEA molecule which is commonly found in proteins with O-glycosylation. Each Ser/Thr-rich repeat contains 1 Ser-Gly-motif which could potentially serve as an attachment site of glycosaminoglycan side-chains. However, digestions with glycosidases fail to demonstrate the presence of glycosaminoglycans. Instead, it is suggested that the size heterogeneity of the 114/A10 molecule is the result of differential post-translational modification with sialylated O-linked carbohydrates, which are probably present in the repeats. MOLECULAR MASS
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Ligands | ||||||||||||||
Function | ||||||||||||||
| The tissue distribution has prompted speculation that 114/A10 might play a regulatory role in the cellular response to IL-3. Further speculation has concerned the group of Arg residues, between the 1st and 2nd EGF-domains, that may be a site for cleavage by proteases. This would release a soluble product, containing the Ser/Thr-repeats and 1 EGF-domain, which might function as a cytokine. | ||||||||||||||
Comments | ||||||||||||||
Database accession numbers | ||||||||||||||
Revised June 25, 2008
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