114/A10 
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 1 glycoprotein
Hematopoietic Cell, Mouse
Myeloid Cell
Progenitor Cell
Erythroid Cell
95 / 305

Expression
The 114/A10 antigen is expressed on mouse hematopoietic cells and cell lines that are responsive to IL-3, including primary erythroid, myeloid and multi-potent progenitors.

Structure
114/A10 is a single chain type1 glycoprotein.  The extracellular N-terminus is comprised of 8 highly conserved Ser/Thr-rich repeats of about 27 residues each, followed by 3 complete epidermal growth factor (EGF)-domains and 1 truncated EGF do-ain.  Between EGF-domains 1 and 3 there is a region of 120 residues with similarity to the recently defined SEA molecule which is commonly found in proteins with O-glycosylation.  Each Ser/Thr-rich repeat contains 1 Ser-Gly-motif which could potentially serve as an attachment site of glycosaminoglycan side-chains.  However, digestions with glycosidases fail to demonstrate the presence of glycosaminoglycans.  Instead, it is suggested that the size heterogeneity of the 114/A10 molecule is the result of differential post-translational modification with sialylated O-linked carbohydrates, which are probably present in the repeats.

MOLECULAR MASS
Cell Type Unreduced Reduced
Mean 150 kDa
Cell type variation 100 - 300 kDa



Ligands


Function
The tissue distribution has prompted speculation that 114/A10 might play a regulatory role in the cellular response to IL-3.  Further speculation has concerned the group of Arg residues, between the 1st and 2nd EGF-domains, that may be a site for cleavage by proteases.  This would release a soluble product, containing the Ser/Thr-repeats and 1 EGF-domain, which might function as a cytokine.

Comments


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
MouseA33533P19467J04634
Antibodies

Revised June 25, 2008


Contact us: Webmaster |  509-335-9515 | Accessibility | Copyright | Policies
College of Veterinary Medicine Washington State University, Pullman, WA, 99164-7010 USA
Copyright 1995-2003 Washington State University