CD205 LY75 (lymphocyte antigen 75), DEC-205
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 1 glycoprotein
Dendritic Cell
Lymph Node
Peyer's Patch
Langerhans Cell
Epithelial Cell
Lymphocyte
Peripheral blood
Thymus
Spleen
Colon
205 / 205

Expression
CD205 is expressed on dendritic cells.  CD205 is expressed on CD11c+ and CD11c- blood dendritic cells but is not restricted to dendritic cells but is rather widely distributed in blood leukocyte populations.  The regulation of its expression on dendritic cells and other cells may contribute to the control of its function.  CD205 is expressed in a lesser density on the surface of T and B cells.  CD205 in the mouse is recognized by the monoclonal antibody NLDC-145.  Using this antibody the antigen was originally reported to be expressed on dendritic cells in the spleen, lymph nodes, Peyer's patches, thymic medulla and skin of Langerhans cells and on epithelial cells in the thymic cortex and intestinal villi.  A rabbit polyclonal antibody raised against purified CD205 has subsequently detected at low levels of expression on mature B cells, granulocytes, thioglycollate-elicited macrophages, thymocytes, mature T cells from spleen and lymph node, bone marrow stromal cells, the epithelia of the lung and in brain capillaries.  Expression is also in thymus, colon, and peripheral blood lymphocytes.

Structure
MOLECULAR FAMILY NAME: Belong to the C-type lectin family.

CD205 is a single-pass type 1 glycoprotein.  It contains a signal peptide, an 1639 aa extracellular domain which contains a ricin B-type lectin domain, a N-proximal fibronectin type-2 domain followed by 10 C-type carbohydrate recognition-like lectin domains and 14 N-linked glycosylation sites, a 25 aa transmembrane domain and a 31 aa cytoplasmic domain containing a tyrosine-based motif used to mobilize the clathrin-endocytic pathway.  The cDNA for CD205 encodes a 1,722 aa with a molecular mass of 198 kDa.  CD205 is a putative antigen uptake receptor for mannosylated antigens.  The molecule shows an overall sequence similarity to the macrophage mannose receptor, the M-type receptor for secretory phospholipases A2 and a 4th, widely expressed, memory of this C-type lectin family.  The N-terminus of  CD205 has been confirmed by protein sequencing.  Human CD205 is 77% identical to the mouse protein with completely conserved 61 cysteines and 14 potential N-glycosylation sites.  The mannose binding motif EPN is found in human CD205 but it is conserved in the mouse protein. 

MOLECULAR MASS
Cell Type Unreduced Reduced
205 kDa

POST-TRANSCRIPTIONAL MODIFICATION

Alternative splicing yields 3 different isoforms.  Isoforms 2 and 3 are produced in Hodgkin's and Reed-Sternberg cells by a transcriptional control mechanism which cotranscribe an mRNA containing CD205 and CD302 prior to generating the intergenically spliced mRNA to produce CD205/CD302 fusion proteins.  The cell surface expression of CD205 is unchanged in blood dendritic cells upon activation but mRNA levels in these dendritic cells increases markedly suggesting that there is a large intracellular pool or post-transcriptional regulation.

POST-TRANSLATIONAL MODIFICATION
 
CD205 is N-glycosylated.

Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH CD205

CD205 binds a variety of antigens, presumably through carbonhydrate-binding lectin domains.

Function
CD205 is a recyled endocytic receptor that directs bound antigens from the extracellular space to a specialized antigen-processing machinery for presentation by MHC class I and causes reduced proliferation of B lymphocytes.  CD205 has a fibronectin II domain and carbohydrate recognition like domains.  It may play a role in the initiation of immune response.  Data suggest that CD205 may function as a multi-functional molecule involved not only in antigen uptake and processing but also in cell-cell and/or cell-tissue interactions.  Electron microscopy studies using gold-labeled monoclonal and polyclonal anti-CD205 antibodies have shown that dendritic cells rapidly internalize the antigen by means of coated pits and vesicles.  Dendritic cells treated with rabbit anti-CD205 antibodies were also found to induce activation of rabbit IgG-specific T cell clones more efficiently than cells treated with non-immune rabbit IgG.  Together these data suggest that CD205 contributes to antigen presentation.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD205 IN INTACT ANIMAL

Isoform 2 and 3 are expressed in malignant Hodgkin's lymphoma cells called Hodgkins and Reed Sternberg cells. 

Comments
MOLECULAR INTERACTIONS -
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD205: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD205: No information.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 4065O60449
MouseU19271
MouseAF011333
Antibodies
CC98   View Reactivity

Revised June 25, 2008


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