CDw210a IL-10Rα (interleukin 10 receptor α)
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 1 glycoprotein
Hematopoietic Cell
T Cell
B Cell
NK Cell
Monocyte
Macrophage
Lymphocyte
Chemokine Receptor
Spleen
Thymus
63 / 63

Expression
CDw210α is highly expressed mainly on hematopoietic cells including T and B cells, NK cells, monocytes, macrophages and on large granular lymphocytes.  Expression is in spleen, thymus and PBMC.  Weak expression is in pancreas, skeletal muscle, brain, heart and kidney.  Intermediate levels of expression is in placenta, lung, and liver. 

Structure
MOLECULAR FAMILY NAME: Belongs to the type-2 cytokine receptor family.

CDw210α is a single-pass type-1 578 aa glycoprotein.  It contains an extracellular domain which contains 2 fibronectin type-3 domains, a transmembrane domain and a 318 aa cytoplasmic domain.  The WSXWS motif characteristic of class 1 cytokine receptors is absent from both extracellular subdomains.  The cytokine receptor family includes CD119 (IFNgR), CD118 (IFNα/βR), CD142 tissue factor and viral CD119 orthologues.  The human IL-10R has been identified as a 90 kDa-110 kDa protein by crosslinking experiments.

MOLECULAR MASS
Cell Type Unreduced Reduced
90 - 110 kDa

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION: No information.


Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH CDw210α

The human CDw210α binds IL-10 with a high affinity of kDa = 200-250 pM and the soluble extracellular portion of CDw210α can bind IL-10.  The functional receptor appears to be a tetramer containing two molecules.

Function
The protein encoded by CDw210α is the receptor for IL-10 and binds IL-10 with a high affinity.  CDw210α is primarily a downregulator of activation and the protein is structurally related to interferon receptors.  It has been shown to mediate the immunosuppressive signal of IL-10, and thus inhibits the synthesis of proinflammatory cytokines.  This receptor is reported to promote survival of progenitor myleoid cells through insulin receptor substrate-2/PI 3-kinase/AKT pathway.  Activation of CDw210α binds IL-10 which leads to tyrosine phosphorylation of JAK1 and TYK2 kinases and initiates a cascade through the JAK/STAT pathway.  CDw210α is involved in cell-signaling and immune regulation.  The capacity of neutrophils to respond to IL-10 by Stat3 tyrosine phosphorylation, SOCS-3 expression and modulation of cytokine production is dependent on the level of expression of IL-10R1.  Binding of IL-10 to CDw210α inhibits cytokine synthesis by activated T and NK cells, monocytes and macrophages and also blocks the accessory cell function of macrophages.  IL-10 is primarily a downregulator of activation, inhibiting production of IFN-γ by T cells, monocytes and NK cells.  IL-10 also inhibits dendritic cell function, through binding to the IL-10 receptor on dendritic cells.  Il-10 can co-stimulate proliferation of NK cells induced by IL-2 and can co-stimulates human B cell proliferation and differentiation and synergizes with TGFb to stimulate IgA production.  Mouse IL-10 enhances the proliferation of thymocytes, T and mast cells, upregulates MHC class II expression on B cells and sustains the viability of mouse B and mast cell lines in vitro.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CDw210α IN INTACT ANIMAL

Cytomegalovirus (CMV) and Epstein-Barr virus (EBV) make viral IL-10 analogs that bind to the IL-10 receptor.

Comments
MOLECULAR INTERACTIONS -
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CDw210α: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CDw210α: No information.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 3587Q13651
MouseL12120
Antibodies

Revised June 25, 2008


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