CD174 

FUT3 (fucosyltransferase 3 (galactoside 3(4) L fucosyltransferase, Lewis blood group), LeY

Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Carbohydrate
Stem Cell
Epithelial Cell
Lectin
Stomach
Colon
Small intestine
Kidney
Lung
Prostate
Pancreas
Hematopoietic Progenitor Cell
Leukemia cell
Breast
Cancer cell
170 / 170

Expression
CD174 is expressed to be restricted on normal and tumorous epithelial cells.  CD34+ leukemia cell lines, CD34+ hematopoietic progenitor cells and stem cells express CD174 and CD34+ leukemic cell lines, while CD34- cell lines and mature lymphocytes lack CD174.  Overexpression of CD174 occurs in a wide variety of carcinomas, including breast, prostate, pancreas, and non-small cell lung cancers.  High expression is found in stomach, colon, small intestine, lung, kidney and to a lesser extent in salivary gland, bladder, uterus and liver. 

Structure
MOLECULAR FAMILY NAME: Belongs to the Lewis blood group family.

CD174 is the difucosylated form of the type-2 precursor chain carbohydrate which is found on blood group Lewis Y oligosaccharides of glycolipids and glycoproteins.  It is a product of epistatic interaction of  a1,2 and a1,3 fucosyltransferases encoded by the H gene and the 
α 1,3 fucosyltransferases encoded by the LeX gene.  CD174 is present either in the plasma membrane linked to glcolipids or to surface receptors such as members of the Erb family.  In epithelial ovarian cancer, the major carrier proteins of CD174 are CA125 and MUC1.  In CD34+ hematopoietic stem cells, the major carrier of CD174 is a 170 kDa glycoprotein.

MOLECULAR MASS

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION

CD174 is glycosylated.


Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH CD174: No information.

Function
The function of CD174 is unknown but it is reported that CD174 is implicated as a cofactor to pro-coagulant Y activity of cancer cells and may play a role in early commitment to apoptosis.  In both in vitro and in vivo studies, soluble CD174 was found to be a potent angiogenic mediator.  It may promote cell migration.  Unlike CD15 which first appears on promyelocytes and remains well expressed thereafter, CD174 is expressed in promyelocytes but is absent in more mature hematapoietic cells.  CD174 is a specific marker for CD34+ hematopoietic progenitor cells and CD34+ leukemic cell lines.  Anti Lewis Y mAb conjugated with doxorubicin is being trialed for the therapy of epithelial tumors.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD174 IN INTACT ANIMAL

The selective increase in CD174  expression by synovial fluid granulocytes from patients with rheumatoid arthritis, spondyloarthritis and osteoarthritis suggests a role in granulocyte trafficking and inflammation responses.  CD174 may also be involved in the homing process of hematopoietic stem cells to the bone marrow and may play an important role in liver metastases of colon carcinoma.  CD174 is a specific marker for CD34+ hematopoietic progenitor cells and CD34+ leukemic cell lines.  Anti Lewis Y mAb conjugated with doxorubicin is being trialed for the therapy of epithelial tumors


Comments
MOLECULAR INTERACTIONS -
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD174: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD174: No information.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 2525P21217
Antibodies

Revised June 25, 2008


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