CD203c ENPP3 (ecto-nucleotide pyrophosphatase phosphodiesterase), PDNP3, PD-Iβ
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 2 glycoprotein
Basophil
Mast Cell
Uterus
Hematopoietic Progenitor Cell
Glial Cells
Pancreas
Small intestine
Colon
Liver
Prostate
130 / 130
270 / 270

Expression
CD203c is expressed in the uterus, glioma cells, prostate, on basophils, mast cells and CD34+ hematopoietic progenitor cells.  Expression is on tumor tissues.  In rat expression is reported in a subset of immature glial cells and in the alimentary tract.  The corresponding protein is also detected in the pancreas, small intestine, colon, and liver. 

Structure
MOLECULAR FAMILY NAME: Belongs to the ecto-nucleotide pyrophosphatase/phosphodiesterase enzyme family.

CD203c is a single-pass type-2 875 aa glycoprotein.  It contains an 833 aa extracellular domain which contains 2 membrane proximal cysteine-rich somatomedin B-like domains which are cysteine-rich near the transmembrane domain, a RGH cell adhesion motif, a phosphodiesterase-1 catalytic site, an EF-hand motif and several potential N-linked glycosylation sites, a transmembrane domain and a C-terminal cytoplasmic domain.  It belongs to a family of ecto-nucleotide pyrophosphatase/phosphodiesterase (E-NPP) enzymes that catalyze the hydrolysis of oligonucleotides, nucleoside phosphates and NAD.  Other family members include the plasma cell antigen ENPP1 (PC-1) and ENPP2 (autotaxin).  CD203c is organized into 25 exons and 24 introns.  Exons 1 and 2 encode the cytoplasmic domain, exons 3 and 4 encode the transmembrane domain, exons 5 and 6 encode the somatomedin B-like domain, exon 8 encodes the putative enzymatic catalytic domain, and exon 24 encodes the EF hand-like motif.

MOLECULAR MASS
Cell Type Unreduced Reduced
KU-812 cells 270 kDa 130 and 150 kDa

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION

CD203c has several potential glycosylation sites.


Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH CD203c

CD203c associates with nucleotides.

Function
CD203c is a multi-functional ectoenzyme that may be involved in the clearance of extracellular nucleotides.  CD203c is capable of cleaving a variety of phosphodiester and phosphosulfate bonds present in deoxynucleotides, nucleotide sugars and NAD. 

BIOCHEMICAL ACTIVITY

CD203c belongs to a family of E-NNP enzymes that catalyze the hydrolysis of oligonucleotides, nucleoside phosphates and NAD.  E-NPP possess ATPase EC3.6.1.3 and ATP pyrophosphatase EC 3.6.1.9 and cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars and NAD. They consist of a family of 3 closely related proteins, E-NPP1, E-NPP2, and E-NPP3.  CD203c's role may be to clear extracellular nucleotides.

DISEASE RELEVANCE AND FUNCTION OF CD203c IN INTACT ANIMAL

CD203c is expressed in glioma cells and is strongly expressed by colon carcinoma cells and is associated with malignant subversion and invasion.


Comments
MOLECULAR INTERACTIONS -
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD203c: No information.

SUBSTRATES
Substrate Comment
Nucleoside triphosphates
Nucleoside diphosphates
cAMP
NAD

ENZYMES WHICH MODIFY CD203c: No information.

ADDITIONAL INSIGHTS

CD203c expression in the uterus is restricted to apical, lumen-orientated membrane areas in glandular regions.   The E-NPP family has at least 3 members, and they are named in order of discovery, E-NPP1, E-NPP2, and E-NPP3.  The 1st family member is the plasma cell antigen PC-1, also known as PC.1, PCA1, PCA-1, E-NPP1, NPP1, PDNP1, M6S1, NPPg , and NPPS.  In order to conform to their nomenclature rules, the Human Gene Nomenclature Committee designated this gene ENPP1 in February 2000.  The 2nd family member is known as Autotaxin, also known as ATX, PD-1a, PDIALPHA, NPPa and PDNP2.  This gene was designated ENPP2 in February 2000.  The 3rd family member is known as B10, also known as gp130RB13-6, bovine intestinal phosphodiesterase, PD-1b, PD-Ib and PDNP3.  This gene was designated ENPP3 in February 2000 and CD203c by the 7th Workshop.



Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 5169O14638
RatZ47987
RatD30649
Antibodies

Revised June 25, 2008


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