CD245 p220/240, DY12, DY35
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Unknown
T Cell
Lymphocyte
Monocyte
Granulocyte
Platelet
B Cell
NK Cell
Peripheral blood
Myeloid Cell
220 / 220
250 / 250

Expression
HLDA/HCDM Workshop lists CD245 as not cloned.  Any information below on this CD was obtained from a previous Workshop.

CD245 is expressed on all resting peripheral blood lymphocytes(PBL), monocytes, granulocytes, platelets and NK cells.  Erythrocytes are not stained.  The mAbs react with NK and myeloid cell lines.  T cell lines, such as Molt4, Jurkat, HPB-ALL, were weakly or not stained.  Thymocytes corresponding to different stages of T cell differentiation are all CD245 negative.  High level expression of CD245 occurs in monocytes and IL-2-dependent T cell lines but is weak on T cells, thymocytes, granulocytes and platelets.  The mAbs while not staining EBV-transformed B cells bind to mantle zone B cells, B-CLL and MCL.  The level of expression on PBL does not markedly change during short term PHA stimulation of PBL.  However, between 72 hours and 7 days of PHA activation, population of blasts express significantly higher levels of CD245.  Expression is reported on Ewing sarcoma and osteosarcoma cell lines and weaker on a rhabdomyosarcoma cell line. 

Structure
MOLECULAR FAMILY NAME

CD245  is a transmembrane glycoprotein and has a pan leukocyte type of expression of unknown structure. 

MOLECULAR MASS
Cell Type Unreduced Reduced
220-250 kDa

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION: No information.


Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH CD245: No information.

Function
CD245 is involved in signal transduction and co-stimulation of T and NK cells.  A weak associated phosphate activity was detected in the immunoprecipitates from the NK cell line but not from PBL.  MAb crosslinking of CD245 augments the proliferation of PBL in the presence of suboptimal concentrations of anti-CD2 mitogenic mAbs.  Anti-CD3 induced T cell proliferation is unaltered in the presence of anti-CD245.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD245 IN INTACT ANIMAL: No information.

Comments

MOLECULAR INTERACTIONS -
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD245: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD245: No information.



Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
Antibodies

Revised June 25, 2008


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