CD248 Endosialin,  CD164L1 (CD164 sialomucin-like protein), TEM1
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 1 glycoprotein
Fibroblast
Tumor Cell
Endothelial Cell
Endothelium
Skeletal muscle
175 / 175

Expression
CD248 is considered to be a tumor endothelial cell marker that is expressed by fibroblast-like cells near the meninges and α-smooth muscle cells in some vessels but not in normal blood vessels.  A recent study has suggested that CD248 is not expressed by tumor endothelial cells and is only expressed by stomal fibroblasts and a subset of pericytes associated with tumor vessels.  Expression is absent or barely detectable in normal lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. 

Structure
MOLECULAR FAMILY NAME: Belongs to the C-type lectin family.

CD248 is a single-pass type-1 757 aa glycoprotein.  It contains a signal peptide, an extracellular domain which contains 5 globular domains (3 EGF domain, a C-type lectin domain and a Sushi (CCP/SCR) domain and a mucin-like region, a transmembrane domain and a short intracellular cytoplasmic domain which contains a 360 aa N-terminal which are homologous to thrombomodulin and the complement receptor C1qRp.   CD248 carries sialylated O-linked oligosaccharides which is reduced to 120 kDa by sialidase treatment or reduced to 95 kDa with additional O-glycanase treatment. 

MOLECULAR MASS

POST-TRANSCRIPTIONAL MODIFICATION

Alternative splicing yields 2 isoforms.

POST-TRANSLATIONAL MODIFICATION

CD248 is heavily O-glycosylated with sialylated oligosaccharides and N-glycosylated.

Ligands
LIGANDS AND MOLECULE ASSOCIATED WITH CD248: No information.

Function
The structural protein similarities between CD141 (thrombomodulin), CD93 and CD248 suggests a possible role for CD248 in cell-cell interactions, particularly during tumor angiogenesis and metastasis.  CD248 is a receptor involved in regulating blood coagulation, and complement receptor C1qRP.(* See available information under CD-C1qRP).  The exact function of CD248 has yet to be elucidated.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD248 IN INTACT ANIMAL

Expression of CD248 correlates with high tumor grade and aggressive progression of tumors.


Comments
MOLECULAR INTERACTIONS-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD248: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD248: No information.

ADDITIONAL INSIGHTS

For further information see Opavsky, R. et al (2001) J. Biol. Chem. 276: 38795-38807.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene57124Q9HCU0
Antibodies

Revised June 25, 2008


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