CD258 

TNFSF14 (tumor necrosis factor ligand superfamily member 14), HVEML (herpes virus entry mediator ligand), LIGHT

Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 2 glycoprotein
Dendritic Cell
T Cell
Monocyte
Spleen
Brain
29 / 29

Expression

CD258 is expressed on activated T cells, monocytes and dendritic cells derived by in vitro culture but not by dendritic cells isolated fresh from blood.  Expression is predominantly in the spleen and brain.  Low expression is in resting blood and tissue leukocytes and is upregulated on activation.  Weak expression is on peripheral lymphoid tissues, heart, placenta, liver, lung, appendix, and kidney but no expression is seen in fetal tissues, endocrine glands, nonhematopoietic tumor lines and most cancer cell lines do not express CD258.



Structure
MOLECULAR FAMILY NAME: Belongs to the tumor necrosis factor superfamily.

CD258 is a single-pass type-2 240 aa glycoprotein.  It contains an 181 aa extracellular domain which contains 1 N-glycosylation site, a  22 aa transmembrane domain and a 37 aa N-terminal cytosolic cytoplasmic domain. 

MOLECULAR MASS

POST-TRANSCRIPTIONAL MODIFICATION

Alternative splicing yields 2 different isoforms. The soluble form of isoform 1 is derived from the membrane form by proteolytic processing and is secreted.  Isoform 2 is cytoplamic.

POST-TRANSLATIONAL MODIFICATION

CD258 is N-glycosylated.


Ligands
LIGANDS AND MOLECULE ASSOCIATED WITH CD258 

CD258 binds two receptors both members of the TNF receptor family, CD270 (HVEM) and LTbR.

Function
Redundnacy in function and receptor binding in the TNF family makes it difficult to work out the physiological function of each member.  CD258 is the ligand for herpes virus entry mediator (TNFRSF14), lymphotoxin β receptor and decoy receptor 3 (DcR3/TR6).  CD258 can trigger apoptosis in tumor cells as well as cell activation by signaling through TRAF3.  This protein has a role in the immune system and can act as a costimulatory for stimulating T cell proliferation and cytokine secretion preferentially TH1 cytokines.  The protein is also reported to prevent tumor necrosis factor α mediated apoptosis in primary hepatocyte.  CD258 induces activation of dendritic cells, indicated by upregulation of costimulatory and adhesion molecules and increased antigen presentation.  It is also thought to play a role in the maturation of dendritic cells and the regulation of apoptosis. CD258 also appears to function in thymic repertoire selection and in aspects of the inflammatory response. 

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION WHICH REGULATE TRANSCRIPTION OF CD258

The protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. 

Comments
MOLECULAR INTERACTIONS-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD258: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD258: No information.

ADDITIONAL INSIGHTS

For further information see Wang, J. et al (2001) J. Clin. Invest. 108: 1771-1780.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 8740O43557
Antibodies

Revised June 25, 2008


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