CD255 TNFSF12 (tumor necrosis factor (ligand) superfamily, member 12, TNF-related weak inducer of apoptosis, 
TWEAK,  APO3L
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 2 glycoprotein
Monocyte
Heart
Pancreas
Brain
Small intestine
Lung
Ovary
Prostate
Spleen
Lymph Node
Skeletal muscle
Endothelial Cell
Fibroblast
Peripheral blood
Lymphocyte
Nervous system
T Cell
Thymus
Carcinoma Cell
Colon
Tumor Cell
18 / 18
35 / 35

Expression
CD255 is expression in IFNγ stimulated peripheral blood lymphocytes, monocytic cell lines, endothelial cells, smooth muscle cells and  fibroblasts.  Expression is at high levels in the central nervous system, adult heart, pancreas and skeletal muscle.  Expression has also been detected in spleen, lymph node, thymus, T cells, in colorectal carcinoma cell lines, first and third trimester placenta and various tumor cells.  Expression is high in the brain, colon, small intestine, lung, ovary, prostate and appendix.  CD255 is expression in IFNγ stimulated peripheral blood lymphocytes. 

Structure
MOLECULAR FAMILY NAME: Belongs to the tumor necrosis factor superfamily.

CD255 is a single-pass type-2 249 aa glycoprotein.  It contains a 21 aa signal sequence, a 207 aa C-terminal extracellular domain which contains a receptor-binding subdomain predicted to be a β-pleated sheet structure, a single N-linked glycosylation site and a 90-93 aa proleolytic cleavage, a 21 aa hydrophobic transmembrane domain which contains a link to the receptor binding domain via a stalk region containing a high proportion of basic amino acids and a 21 aa N-terminal cytoplasmic domain which contain a putative serine phosphorylation site.  Ligand binding induces the formation of CD255 homotrimers.  CD255 is a cytokine that belongs to the TNF ligand family and is the ligand for the FN14/TWEAKR receptor.

MOLECULAR MASS

POST-TRANSCRIPTIONAL MODIFICATION

Alternative splicing yields 2 different isoforms.  There is a membrane form of 18 kDa and a soluble isoform of 30-35 kDa and 156 aa which comprises the receptor binding portion of the ectodomain results from proteolytic cleavage by furin

POST-TRANSLATIONAL MODIFICATION: No information.

Ligands
LIGANDS AND MOLECULE ASSOCIATED WITH CD255

CD255 binds CD266.

Function
CD255 has overlapping signaling functions with TNF, but displays a much wider tissue distribution.  It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner.  CD255 is able to weakly induce apoptosis in many tissues and cell types and is involved in the IFNγ stimulated killing of CD255 sensitive tumor cells.  In contrast it mediates NK-κB activation, migration and proliferation of endothelial cells, promotes angiogenesis including neovascularization of tumors and is involved in the induction of inflammatory cytokine secretion.  Both the soluble and membrane bound forms are biologically active.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD255 IN INTACT ANIMAL: No information.

Comments
MOLECULAR INTERACTIONS-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD255: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD255: No information.

Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 8742O43508
Antibodies

Revised June 25, 2008


Contact us: Webmaster |  509-335-9515 | Accessibility | Copyright | Policies
College of Veterinary Medicine Washington State University, Pullman, WA, 99164-7010 USA
Copyright 1995-2003 Washington State University