CD272 BTLA (B and T lymphocyte attenuator precursor)
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 1 glycoprotein
Th1 Cell
T Cell
B Cell
Spleen
Lymph Node
Lymphocyte
Macrophage
Dendritic Cell
Bone Marrow
33 / 33

Expression
CD272 expression is induced during activation of T and B cells but expressed on Th1 cell but not on Th2 cells.  Expression is on all mature lymphocytes, splenic macrophages, bone marrow-derived dendritic cells, in lymph node and spleen.        

Structure
MOLECULAR FAMILY NAME: Belongs to the immunoglobulin gene family.

CD272 is a single-pass type-1 259 aa glycoprotein.  It contains a 30 aa signal sequence, an 127 aa extracellular domain which contains an Ig-like V-type domain and 3 potential N-linked glycosylation sites, a 21 aa transmembrane domain and an 111 aa intracellular C-terminal cytoplasmic domain which contains 2 immunoreceptor tyrosine-based inhibitory motifs (ITIMs) which is required for association with phosphatases SHP-1 and SHP-2 and a third motif which is a Grb-2 recognition consensus.  Analysis demonstrated a surface expression of a transmembrane protein and showed expression of a glycosylated protein that could be phosphorylated on tyrosines in the ITIMs.  

MOLECULAR MASS

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION

CD272 is phosphorylated on Try residues by TNFRSF14 and by antigen receptors crosslinking, both inducing association with PTPN6/SHP-1 and PTPN11/SHP-2.  CD272 is N-glycosylated.

Ligands
LIGANDS AND MOLECULE ASSOCIATED WITH CD272

CD272 binds CD270 (LIGHT-R, HVEM).

Function
CD270 ligation of CD272 induces tyrosine phosphorylation of the CD272 cytoplasmic domain, recruitment of phosphatases SHP-1 and SHP-2 and inhhibitory signaling which results in attenuation of T-cell activation.  CD272 interacts with the B7 homolog 4 (B7HA).  It is a lymphocyte inhibitory receptor on T lymphocytes which inhibits lymphocytes during immune response and has  similarities to CD152 (CTLA-4) and CD279 (PD-1).  Knockout mice have stronger B- and T-cell responses than wild-type mice and increased susceptibility to autoimmunity.  
  
BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD272 IN INTACT ANIMAL: No information.

Comments
MOLECULAR INTERACTIONS-
PROTEIN AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD272: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD272: No information.

ADDITIONAL INSIGHTS

For further information see Watanabe, N. et al (2003) Nat. Immunol. 4: 670-679.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrez151888Q7Z6A9
Antibodies

Revised June 25, 2008


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