CD294 GPR44 (G protein-coupled receptor 44), CRTH2
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 3 glycoprotein, 7 span
Eosinophil
Basophil
Th2 Cell
T Cell
Heart
Brain
Gut
43 / 43

Expression
CD294 is widely distributed outside the immune system including gut, heart and brain.  Within the immune system, CD294 shows differential expression and is particularly associated with a subset of T cells that on stimulation produce preferentially IL-4, IL-5 and IL-13, referred to as Th2 cells.  Expression is also on eosinophils and basophils, which along with Th2 cells are involved in allergic disease.



Structure

MOLECULAR FAMILY NAME: Belongs to the G-protein receptor family.

CD294 is a multi-pass type-3 7-span 395 aa glycoprotein.  It contains an extracellular domain which contains a N-terminal region and 2 potential N-glycosylation sites, a transmembrane domain and a long cytoplasmic C-terminal domain which has sites for phosphorylation by protein kinase C.  The homology murine CD294 has a 77% amino acid identity.

MOLECULAR MASS

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION

CD294 has 2 potential N-glycosylation sites.



Ligands
LIGANDS AND MOLECULE ASSOCIATED WITH CD294

CD294 binds prostaglandin D2 (PGD2).

Function
CD294 resembles the chemokine receptors and receptors for other chemoattractant stimuli, but its ligand appears to be prostaglandin D2 (PGD2).  These proteins mediate signals to the interior of the cell via activation of heterotrimeric G proteins that in turn activate various effector proteins, ultimately resulting in a physiologic response.  CD294 is thought to be a potential marker of Th2 cells and to play an important role in the regulation of immune allergic inflammatory response through chemotactic and  induced stimulatory effects on Th2 cells, eosinophils and basophils migration.  CD294 stimulates various changes in cells expressing CD294, the responses depend on the cell type.  In general, the responses can be described as activation but the physiological role is not yet clear. 

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD294 IN INTACT ANIMAL: No information.

Comments
MOLECULAR INTERACTIONS-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD294: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD294: No information.

ADDITIONAL INSIGHTS

For further information see Nagata, K. et al (1999) J. Immunol. 162: 1278-1286.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene11251Q9Y5Y4
Antibodies

Revised June 25, 2008


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