CD296 ART1 (ADP-ribosyltransferase 1), ART2
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
GPI anchor
T Cell
Muscle Cell
Epithelial Cell
NK Cell
Neutrophil
Heart
37 / 37

Expression
CD296 is expressed on neutrophils, epithelial and skeletal muscle cells and NK and T cell subsets.  Expression is also in the heart and skeletal muscle.

Structure
MOLECULAR FAMILY NAME: Belongs to the Arg-specific ADP-ribosyltransferase family.

CD296 is 273 aa GPI-anchor protein.  It contain  an extracellular domain which contains a C-terminal which contains a β-sheet rich domain and thought to be formed by the intersection of three domains and has 2 potential N-terminal glycosylation sites but there is no transmembrane domain or cytoplasmic domain.  Domain 1 and 2 are defined by a conserved arginine and consensus Ser-X-Ser motifs and is involved in stabilizing NAD binding.  Domain 3 contains a catalytic glutamate which is required for NAD hydrolysis.  The N-terminal contains α helices and influences the transfer of ADP-ribose to an acceptor amino acid or to water.  CD296 is an ADP-ribosyltransferase which catalyzes the ADP-ribosylation of arginine residues in a structurally related family of proteins that are expressed on the cell surface or secreted in the extracellular matrix.  This gene was previously designated ART2. 

MOLECULAR MASS

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION

CD296 has 2 potential N-terminal glycosylation sites.

Ligands
LIGANDS AND MOLECULE ASSOCIATED WITH CD296

CD296 associates with PDGFβ, integrins and defensin.

Function
CD296 catalyzes the transfer of a single ADP-ribose group from NAD onto an arginine of a target protein, thereby reversibly modifying the protein's function.  Substrates of CD296 include integrin α7, defensin-1, CD11a, CD18, as well as ADP-ribosylate guanidine containing substrates such as agmatine and arginine methyl ester.  CD296 can also ribosylate small soluble proteins such as the basic fibroblast growth factor (FGF-2).  CD296 is involved in the regulation of cellular metabolism and associated with myocyte differentiation, inflammatory responses and inhibition of proliferation and cytotoxicity by cytotoxic T cells.  

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD296 IN INTACT ANIMAL

Mono-ADP-ribosylation is a posttranslational of proteins that is interfered with a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. 


Comments
MOLECULAR INTERACTIONS-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD296: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD296: No information.

ADDITIONAL INSIGHTS

For further information see Seman, M. et al (2004) Curr. Med. Chem. 11: 857-872.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 417P52961
Antibodies

Revised June 25, 2008


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