CD298 ATP1β3 (Na/K ATPase β3-subunit)
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 2 glycoprotein
Testis
Leukocyte
Nervous system
Nonhematopoietic Cell
32 / 32

Expression
CD298 is expressed on all leukocytes.  Studies with non-hemopoietic cell lines suggest the protein is also expressed by many other tissues.  Expression is high in CNS and testis.

Structure
MOLECULAR FAMILY NAME: Belongs to the ATPase β-chain family.

CD298 is a single-pass type-2 279 aa integral glycoprotein.  It contains a 223 aa extracellular domain containing 2 potential N-linked glycosylation sites, a 21 aa 1 putative transmembrane domain and a 35 aa cytoplasmic domain.  The protein belongs to the family of Na/K and H/K ATPases β chain proteins, and to the subfamily of Na/K -ATPases.  CD298 is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane.  These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle.  This enzyme is composed of three subunits, a large catalytic subunit (α) and a smaller glycoprotein subunit (β) and a γ subunit.  The β subunit regulates, through assembly of α/β heterodimers, the number of sodium pumps transported to the plasma membrane.  The glycoprotein subunit of CD298 is encoded by multiple genes.  This gene encodes a β3 subunit.  A pseudogene exists for this gene.

MOLECULAR MASS

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION

CD298 has 2 potential N-linked glycosylation sites.



Ligands
LIGANDS AND MOLECULE ASSOCIATES WITH CD298

CD298 dimerizes with ATPα1, 2 or 3.

Function
CD298 (N/K ATPase) is a membrane localized enzyme involved in Na/K transport.  Its expression is induced in leukocytes upon activation.  The function of the CD298 is a non-catalytic component to exchange and transport sodium and potassium ions across the cell membrane.  This protein is made up of a catalytic α subunit and a β subunit.  Both subunits are required for the functionally active enzyme.  Three β subunit isoforms have been identified.  The combination of the various α and β subunits is thought to regulate/modify enzymatic activity of the heterodimer.  The specific role of the non-catalytic β-chain is not known. 

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD298 IN INTACT ANIMAL: No information.

Comments
MOLECULAR INTERACTIONS-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD298: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD298: No information.

ADDITIONAL INSIGHTS

For further information see Malik, N. et al (1996) J. Biol. Chem. 271 22754-22758.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 483P54709
Antibodies

Revised June 25, 2008


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