CD301 CLEC10A (C-type lectin domain family 10, member A), MGL, CLECSF14,  HMGL, HML2, HML
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 2 glycoprotein
Dendritic Cell
Macrophage
38 / 38

Expression
CD301 is expressed on macrophages and immature dendritic cells.

Structure
MOLECULAR FAMILY NAME: Belongs to the C-type lectin superfamily.

CD301 is a single-pass type-2 316 aa transmembrane glycoprotein.  It contains a 256 aa extracellular domain which contains a C-type lectin domain and 2 potential N-linked glycosylation sites, a 21 aa transmembrane domain and a 39 aa intracellular cytoplasmic domain contains a single endocytosis signaling motif (YENF) which is required for interaction with clathrin-coated vessels.  Residues 85-176 are predicted to form an α-helical coiled coil and this domain is involved in the formation of trimers.  

MOLECULAR MASS

POST-TRANSCRIPTIONAL MODIFICATION:

Alternative splicing yields 3 different isoforms.

POST-TRANSLATIONAL MODIFICATION

CD301 has 2 potentialy N-linked glycosylation sites.

Ligands
LIGANDS AND MOLECULE ASSOCIATED WITH CD301

CD301 binds to carbohydrates bearing terminal galactose and N-acetylgalactosamine residues.

Function
CD301 is a marker of immature dendritic cells and functions as a receptor-mediated endocytosis for the uptake of glycosylated antigens.  CD301 is involved in macrophage migration and cellular recognition.  Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and in regulating adaptive and innate immune responses.  CD301 may function as a cell surface antigen.  Binding in a calcium-dependent manner occurs to terminal glactose and N-acetylgalactosamine units linked to serine or threonine.  CD301 recognizes terminal galactose and N-acetylgalactosamine units, particularly clusters of truncated O-linked carbohydrate chains such as T and Tn antigens of mucins. 

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD301 IN INTACT ANIMAL

Sugar moieties known as Tn antigens are expressed by a variety of carcinoma cells and it has been demonstrated in vitro that  murine CD301+ cells can capture tumor cells by interacting with tumor cell carbohydrate epitopes.  It is postulated that CD301 might contribute to anti-tumor immunity.  Knockout mice show a deficit in granulation tissue formation.  CD301 has roles in inflammation and immune response.


Comments
MOLECULAR INTERACTIONS-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD301: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD301: No information.

ADDITIONAL INSIGHTS

For further information see Higashi, N. et al (2002) J. Biol. Chem. 277: 20686-20693.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene10462Q81UN9
Antibodies

Revised June 25, 2008


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