|CD301||CLEC10A (C-type lectin domain family 10, member A), MGL, CLECSF14, HMGL, HML2, HML|
|Molecule Type||Antigen Expression||Molecular Weight|
Min / Max
|Non-lineage Restricted Molecule|
Type 2 glycoprotein
|38 / 38|
|CD301 is expressed on macrophages and immature dendritic cells.|
|MOLECULAR FAMILY NAME: Belongs to the C-type lectin superfamily.|
CD301 is a single-pass type-2 316 aa transmembrane glycoprotein. It contains a 256 aa extracellular domain which contains a C-type lectin domain and 2 potential N-linked glycosylation sites, a 21 aa transmembrane domain and a 39 aa intracellular cytoplasmic domain contains a single endocytosis signaling motif (YENF) which is required for interaction with clathrin-coated vessels. Residues 85-176 are predicted to form an α-helical coiled coil and this domain is involved in the formation of trimers.
Alternative splicing yields 3 different isoforms.
CD301 has 2 potentialy N-linked glycosylation sites.
|LIGANDS AND MOLECULE ASSOCIATED WITH CD301|
CD301 binds to carbohydrates bearing terminal galactose and N-acetylgalactosamine residues.
|CD301 is a marker of immature dendritic cells and functions as a receptor-mediated endocytosis for the uptake of glycosylated antigens. CD301 is involved in macrophage migration and cellular recognition. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and in regulating adaptive and innate immune responses. CD301 may function as a cell surface antigen. Binding in a calcium-dependent manner occurs to terminal glactose and N-acetylgalactosamine units linked to serine or threonine. CD301 recognizes terminal galactose and N-acetylgalactosamine units, particularly clusters of truncated O-linked carbohydrate chains such as T and Tn antigens of mucins. |
BIOCHEMICAL ACTIVITY: No information.
DISEASE RELEVANCE AND FUNCTION OF CD301 IN INTACT ANIMAL
Sugar moieties known as Tn antigens are expressed by a variety of carcinoma cells and it has been demonstrated in vitro that murine CD301+ cells can capture tumor cells by interacting with tumor cell carbohydrate epitopes. It is postulated that CD301 might contribute to anti-tumor immunity. Knockout mice show a deficit in granulation tissue formation. CD301 has roles in inflammation and immune response.
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD301: No information.
SUBSTRATES: No information.
ENZYMES WHICH MODIFY CD301: No information.
For further information see Higashi, N. et al (2002) J. Biol. Chem. 277: 20686-20693.
Database accession numbers
Revised June 25, 2008