CD49e ITGA5 (integrin α-5 fibronectin receptor α polypeptide), VLA-5α chain
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 1 glycoprotein
Platelet
Thymocyte
Monocyte
T Cell
B Cell
NK Cell
Dendritic Cell
Endothelia
Osteoblast
25 / 25
135 / 135
155 / 155

Expression
CD49e is expressed on thymocytes, T cells, monocytes, platelets, NK cells, dendritic cells, osteoblasts and endothelia.  Expression is increased on activated and memory T cells.  It is also expressed on very early and activated B cells.

Structure
MOLECULAR FAMILY NAME: Belongs to the integrin a chain family.

CD49e is a single-pass type-1 1008 aa glycoprotein.  It contains a large extracellular domain which contains a seven-fold repeated structure with putative cation binding motifs in 4 C-terminal repeats and 14 potential N-glycosylation sites, a 28 aa transmembrane domain and a cytoplasmic domain.  All integrin α chains have a conserved sequence found in the cytplasmic domain imediately following the transmembrane domain (FYWS)-(RK)-x-G-F-F-x-R, which may be the motif involved in heterodimer formation.  CD49e noncovalently associates with the CD29 to form the VLA-5 (integrin α5/β1) complex.    CD49e is post-translationally cleaved into two disulfide linked units of 135 kDa and 25 kDa. 

MOLECULAR MASS
Cell Type Unreduced Reduced
155 kDa 135 kDa, 25 kDa

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION

CD49e is post-translationally cleaved in the extracellular domain to yield 2 C-terminal  disulfide-linked light and heavy chains that join with β1 to form a fibronectin receptor.         

Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH CD49e

CD49e/CD29 is the fibronectin receptor and binds to the RGD sequence of fibronectin.  CD49e/CD29 has been shown to bind the neural adhesion molecule L1, which is also expressed on some leukocytes.

Function
CD40e is reported to be important in the maintenance of endothelial monlayer integrity along with CD49b. It is involved in adhesion, regulation of cell survival and apoptosis.  It is an integrin that mediates binding of the cell to fibronectin substrata.  It is involved in monocyte migration into the extracellular tissues.  In addition to adhesion, CD49e is known to participate in cell-surface mediating signaling.  CD49e/CD29-mediated binding to fibronectin provides a co-stimulatory signal to T cells.  It also enhances Fcg receptor-and complement receptor-mediated phagocytosis.  VLA-2-mediated binding of monocytes to collagen CD49e/CD29 is involved in monocyte migration into extracellular tissues.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD49e IN INTACT ANIMAL

CD49e null mutation is embryonic lethal with mesodermal abnormalities.

Comments
MOLECULAR INTERACTIONS-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD49e: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD49e: No information.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 3678P08648
MousePL0103P11688X15203
Antibodies
IIA1   View Reactivity
JBS5   View Reactivity
SAM-1   View Reactivity
VC5   View Reactivity

Revised June 25, 2008


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