CD323 JAM-3 (junctional adhesion molecule 3), JAM-C, VE-JAM, FLJ14529
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 1 glycoprotein
Megakaryoblastic Cell
Brain
Kidney
T Cell
Lymphocyte
NK Cell
Endothelial Cell
Platelet
Placenta
43 / 43

Expression

CD323 is widely expressed with the highest expression in placenta, brain and kidney.  There is wide expression in many tissues, cultured endothelial cells, cytotoxic T cells lines and activated T lymphocytes but not in resting cells.  Analysis shows expression on NK cells, platelets, T cells and megakaryoblastic cells.



Structure
MOLECULAR FAMILY NAME: Belongs to the immunoglobulin gene family.

CD323 is a single-pass type-1 279 aa glycoprotein.  It contains a 31 aa signal sequence, a 210 aa extracellular domain which contains 1 Ig-like C2-type domain and 1 Ig-like V-type domain, 2 potential N-linked glycosylation sites and 1 potential O-linked glycosylation site, a 21 aa transmembrane domain and 48 aa intracellular cytoplasmic domain with 3 potential phosphorylation Tyr sites, a putative phosphorylation site for casein III kinase and at the C-terminus, there is a potential binding site for PDZ domains..  CD323 is a member of the subfamily of junction adhesion molecules and is more than 30% identical to CD321 (JAM1) and CD322 (JAM2).  It has been proposed that CD323 is a functional receptor for CD322. 

MOLECULAR MASS

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION

CD323 has 2 potential N-liked glycosylation sites, 1 O-linked glycosylation site and 3 potential phosphorylation sites.

Ligands
LIGANDS AND MOLECULE ASSOCIATED WITH CD323

CD323 interacts with CD322 (JAM2) and CD11b/CD18 (Mac-1) and displays homophilic binding.

Function
CD323 localizes to the inter-endothelial tight junctions and plays a role in neutrophil transmigration across endothelial cells via the interaction of CD323 on the endothelium with CD11b/CD18 (Mac-1) on the surface of neutrophils.  Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets forming continuous seals around cells serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space.  CD323 is localized in the tight junctions between epithelial cells.  CD323 plays a role in lymphocyte transmigration vis homophilic interaction with endothelial cells.  Unlike other proteins in this Ig family, this protein is unable to adhere to leukocyte cell kines and only forms weak homotypic interactions.  CD323 is a member of the junctional adhesion molecule family as a receptor for another member of this family (CD322). 

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD323 IN INTACT ANIMAL

CD323 is a potential target for anti-inflammatory therapy for inflammatory vascular pathologies.


Comments
MOLECULAR INTERACTIONS-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD323: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD323: No information.

Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene83700Q9BX67
Antibodies

Revised June 25, 2008


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