|CD323||JAM-3 (junctional adhesion molecule 3), JAM-C, VE-JAM, FLJ14529|
|Molecule Type||Antigen Expression||Molecular Weight|
Min / Max
|Non-lineage Restricted Molecule|
Type 1 glycoprotein
|43 / 43|
CD323 is widely expressed with the highest expression in placenta, brain and kidney. There is wide expression in many tissues, cultured endothelial cells, cytotoxic T cells lines and activated T lymphocytes but not in resting cells. Analysis shows expression on NK cells, platelets, T cells and megakaryoblastic cells.
|MOLECULAR FAMILY NAME: Belongs to the immunoglobulin gene family.|
CD323 is a single-pass type-1 279 aa glycoprotein. It contains a 31 aa signal sequence, a 210 aa extracellular domain which contains 1 Ig-like C2-type domain and 1 Ig-like V-type domain, 2 potential N-linked glycosylation sites and 1 potential O-linked glycosylation site, a 21 aa transmembrane domain and 48 aa intracellular cytoplasmic domain with 3 potential phosphorylation Tyr sites, a putative phosphorylation site for casein III kinase and at the C-terminus, there is a potential binding site for PDZ domains.. CD323 is a member of the subfamily of junction adhesion molecules and is more than 30% identical to CD321 (JAM1) and CD322 (JAM2). It has been proposed that CD323 is a functional receptor for CD322.
POST-TRANSCRIPTIONAL MODIFICATION: No information.
CD323 has 2 potential N-liked glycosylation sites, 1 O-linked glycosylation site and 3 potential phosphorylation sites.
|LIGANDS AND MOLECULE ASSOCIATED WITH CD323|
CD323 interacts with CD322 (JAM2) and CD11b/CD18 (Mac-1) and displays homophilic binding.
|CD323 localizes to the inter-endothelial tight junctions and plays a role in neutrophil transmigration across endothelial cells via the interaction of CD323 on the endothelium with CD11b/CD18 (Mac-1) on the surface of neutrophils. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets forming continuous seals around cells serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. CD323 is localized in the tight junctions between epithelial cells. CD323 plays a role in lymphocyte transmigration vis homophilic interaction with endothelial cells. Unlike other proteins in this Ig family, this protein is unable to adhere to leukocyte cell kines and only forms weak homotypic interactions. CD323 is a member of the junctional adhesion molecule family as a receptor for another member of this family (CD322). |
BIOCHEMICAL ACTIVITY: No information.
DISEASE RELEVANCE AND FUNCTION OF CD323 IN INTACT ANIMAL
CD323 is a potential target for anti-inflammatory therapy for inflammatory vascular pathologies.
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD323: No information.
SUBSTRATES: No information.
ENZYMES WHICH MODIFY CD323: No information.
Database accession numbers
Revised June 25, 2008