CD49f ITG6 (integrin α 6), VLA-6α chain,α-6 integrin chain, platelet gpIc
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Type 1 glycoprotein
T Cell
Monocyte
Thymocyte
T Lymphocyte
Epithelia
Megakaryocyte
Platelet
Lung
Liver
Spleen
Brain
Ovary
Kidney
30 / 30
120 / 120
140 / 140

Expression
CD49f is expressed on thymocytes, T lymphocytes, monocytes, platelets and megakaryocytes.  Wide expression is on cultured adherent cell lines and both CD49f/CD29 and CD49f/CD104 are expressed on epithelia in non-lymphoid tissues.  Increased expression is found on activated and memory T cells.  CD49f also combines with CD104 to form thea6b4 integrin.  The α isoform is expressed in the lung, liver, spleen and cervix whereas only the β isoform is found in brain, ovary and kidney. 

Structure
MOLECULAR FAMILY NAME: Belongs to the integrin a chain family.

CD49f is a single-pass type-1 1050 aa glycoprotein.  It  contains a 991 aa extracellular domain which contains 9 N-glycosylation sites and 3 putative cation-binding motifs, a 23 aa transmembrane domain and a 36 aa or 54 aa cytoplasmic domain.  CD49f noncovalently associates with the CD29 molecule to form the VLA-6 (integrin α6/β1) complex and with CD104 to form the integrin α6/β4 complex.  All α intregin chains have a conserved sequence found in the cytoplasmic domain immediately following the transmembrane domain, (FYWS)-(RK)-x-G-F-F-x-R, which may be the motif involved in heterodimer formation.

MOLECULAR MASS
Cell Type Unreduced Reduced
140 kDa 120 kDa, 30 kDa

POST-TRANSCRIPTIONAL MODIFICATION

Alternative splicing yields 6 different isoforms.

POST-TRANSLATIONAL MODIFCATION

An 1050 aa or 1068 aa protein post-translationally cleaved into two disulfide-linked units of 120 kDa and 30 kDa.


Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH CD49f

CD49f/CD29a6b1 integrin binds laminin receptor on platelets, invasin and merosin.  CD49f/CD104 (β4) binds laminins especially laminin-5. 

Function
CD49f is a component of an adhesion receptor for laminins, invasin merosin involved in cell adhesion and migration, embryogenesis as well as cell-surface mediated signaling.  It enables interaction between epiyhelial cells and basement membrane during wound healing.  It acts as a laminin receptor and is important for the formation of hemidesmosomes of stratified squamous and transitional epithelial.  CD49f/CD29-mediated T cell binding to laminin provides a co-stimulatory signal to T cells for activation and proliferation.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF CD49f IN INTACT ANIMAL

CD49f is involved in tumor metastasis and is a candidate receptor for papilloma virus.  Knockout mice showed perinatal lethality with blistering of the skin which is the same phenotype seen in integrin CD104 (β4).  CD49f enables interaction between epithelial cells and basment membrane during wound healing.  Defects of CD49f are a cause of epidermolysis bullosa with pyloric atresia autosomal ressive disease.

Comments
MOLECULAR INTERACTIONS-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD49f: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD49f: No information.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 3655P23229
Antibodies
4F10   View Reactivity
BQ16   View Reactivity
G0H3   View Reactivity
NKI-GoH3   View Reactivity

Revised June 25, 2008


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