EDG4 

EDG4 (endothelial differentiation, lysophosphatidic acid G-protein coupled receptor, 4)

Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Multi-pass
Leukocyte
Peripheral blood
Testis
T Cell

Expression
EDG4 is expressed in testes and peripheral blood leukocytes with lower expression in pancreas, spleen, thymus and prostate.  Little or no expression is in heart, brain, placenta, ling, liver sketal muscle, kidney, ovary, small intestine or colon.  RT-PCR and Western blot analysis showed that CD4+ T cells and B cells but not CD8+ T cells express EDG4.  Stimulation of CD4+ but not CD8+ T cells in the presence of LPA or monoclonal anti N-terminal EDG4 suppressed IL-12 secretion.

Structure
MOLECULAR FAMILY NAME: Belongs to the G-protein coupled receptor family.

EDG4 is a multi-pass 382 aa glycoprotein.  It contains an extracellular domain which contains potential N-linked glycosylation sites in the N-terminus, 7 hydrophobic transmembrane domains and a cytoplasmic domain which contains potential serine/threonine kinase phosphorylation sites.  EDG4 shares a 46% amino acid identity with EDG2.

MOLECULAR MASS

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION: No information.

Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH EDG4: No information.

Function
EDG4 is a receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities.  Functioning of the LPA receptor contributes to Ca2+ mobilization, a critical cellular response to LPA in cells throught association with Gi and Gq proteins.  It seems to be coupled to families of heteromeric G proteins.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF EDG4 IN INTACT ANIMAL

A cDNA clone hasa guanine nucleotide deletion that causes a frameshift near its C-terminal coding region.  This likely reflects a somatic mutations in the ovary tumor cells from which the cDNA was isolated and may have altered the function of the encoded receptor and contribute to transformation of the original ovary cells that formed a tumor.

Comments
MOLECULAR INTERACTION-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION IN EDG4: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY EDGE4: No information.

Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene 9170Q9HBW0
Antibodies

Revised June 25, 2008


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