HPSE HPSE (heparanase)
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Unknown
Placenta
Spleen
50 / 50

Expression
HPSE is highly expressed in placenta, spleen and weak expression is in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues.

Structure
MOLECULAR FAMILY NAME: Belongs to the glycosyl hydrolase 79 family.

HPSE  is a 543 aa heparanase glycoprotein.  It contains an extracellular domain which contains 6 potential N-linked glycosylation sites, a hydrophic transmembrane domain and a cytoplasmic domain.

MOLECULAR MASS

POST-TRANSCRIPTIONAL MODIFICATION

The cleavage of the 65 kDa isoform leads to the generation of a linker peptide, 8 kDa and 50 kDa product.  The active isoform 8/50 kDa heterodimer, is resistant to degradation.  Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.

POST-TRANSLATIONAL MODIFICATION

HPSE has 6 N-linked glycosylated.  HPSE is proteolytically processed.  Glycosylation of the 50 kDa subunit appears to be essential for its solubility.

Ligands
LIGANDS AND MOLECULES ASSOCIATED WITH HPSE: No information.

Function
Endoglycosidase is a cell surface and extracellular matrix-degrading enzyme.  HPSE cleaves heparan sulfate proteoglycan (HSPGs) into heparan sulfate sides chains and core proleoglycans.

BIOCHEMICAL ACTIVITY: No information.

DISEASE RELEVANCE AND FUNCTION OF HPSE IN INTACT ANIMAL

Due to HSPE contribution and angiogenesis, it is considered to be a potential target for anti-cancer therapies.  The degration of heparan sulfate by heparanase is a key step in the extravasation of tumor cells and migrating leukocytes but is also important in physiologic processes including angiogenesis, would healing and smooth muscle proliferation.

Comments

MOLECULAR INTERACTIONS-
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF HPSE: No information.

SUBSTRATES: No information.

ENZYMES WHICH MODIFY HPSE: No information.



Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
HumanEntrezgene10855Q9Y251
Antibodies

Revised June 25, 2008


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