CD60a GD3
Molecule TypeAntigen ExpressionMolecular Weight
Min / Max
Non-lineage Restricted Molecule
Carbohydrate
Platelet
Pancreas
Epithelium
Keratinocyte
Fibroblast
Muscle, Smooth
Astrocyte
T Cell
Adrenal medullary cell
Melanoma Cell
Thymocyte
Melanocyte
Glial Cells
Peripheral blood
Tumor Cell
90 / 90

Expression
CD60a, which was CDw60 and is now divided into CD60a, CD60b and CD60c.  It is expressed on a subset of T cells, platelets, thymic epithelium, activated keratinocytes, synovial fibroblasts, glomeruli, smooth muscle cells and astrocytes.  CD60a is expressed on cells derived from neuroectoderm, such as the adrenal medullary cells, melanocytes, glial cells, neurons and islet cells of the pancreas.  It is expressed on thymocytes, a subpopulation of peripheral blood T cells and weak expression on B lymphocytes, granulocytes and platelets.  CD60a is a strong marker for malignant melanomas and a few non-melanomatous tumors are positive for CD60a.  Activation of naïve CD4+ T cells results in upregulation of CD60a expression, however, upregulation of CD60a expression does not simply reflect an activated state of the T cell.  CD60a identifies a CD8+ Th cell subset.  CD60a expression seems to be correlated with a CD4+ Th type 2-like cytokine profile.  A very high frequency of CD60a positive T cells is found in synovial fluid of normal and arthritic patients and in cutaneous psoriatic lesions.  Granulocytes contain the highest amount of 9-O-acetyl GD3 CD60b, but are not stained by CD60a.  Keratinocyte CD60a is upregulated by IL-13.



Structure
MOLECULAR FAMILY NAME: Belongs to the carbohydrates family.

CD60a represents the oligosaccharide sequence of the ganglioside GD3 (NeuAc-α2-8NeuAc-α2-3Gal-β1-4Glc-β1-Cer).  CD60a has 4 mAbs to CD60a have been shown to recognize mainly acetylated forms of the ganglioside GD3.  Antibody binding is reduced or abolished following O-deacetylation of the antigen.  The 9-O-acetylated disialosyl group CD60b is predominantly found on ganglioside D3.  The CD60a determinant may further be expressed by T-cell glycoprotein migrating at 90 kDa-94 kDa.

MOLECULAR MASS
Cell Type Unreduced Reduced Comment
PBMC 120 kDa

POST-TRANSCRIPTIONAL MODIFICATION: No information.

POST-TRANSLATIONAL MODIFICATION: No information.


Ligands
CD60a is present on some gangliosides and is immunoprecipitated in association with polypeptides whose molecular weights vary with the tissue examined.  It remains to be determined whether the oligosaccharide/glycolipid is co-valently associated with these polypeptides.

Function
CD60a functions in the regulation of apoptosis and induces mitochondrial permeability transition during apoptosis.  CD60a antibodies deliver co-stimulatory signals in the presence of accessory cells or low doses of phorbol esters, and augment signaling via CD3.  CD60a+ T cells within the CD4 and CD8 subsets provide B cell help and secrete higher levels of IL-4, whereas CD60a-CD8+ cells perform cytotoxic and suppressor functions and are a major source of IFNg .

BIOCHEMICAL ACTIVITY: No Information.

DISEASE RELEVANCE AND FUNCTION OF CD60a IN INTACT ANIMAL

CD60a functions as a positive marker for malignant melanomas and a few non-melanomatous tumors and has been used in therapeutic.  The CD60a antigen is expressed at high levels on 75% of T cells from the synovial fluid of arthritic patients, and on 75% of T cells from cutaneous psoriatic lesions, and may have a role in the pathogenesis of these diseases.



Comments
MOLECULAR INTERACTIONS -
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD60a: No information

SUBSTRATES: No information.

ENZYMES WHICH MODIFY CD60a: No information.


Database accession numbers
AnimalPIRSWISSPROTEMGBL/GENBANK
 
Antibodies

Revised June 25, 2008


Contact us: Webmaster |  509-335-9515 | Accessibility | Copyright | Policies
College of Veterinary Medicine Washington State University, Pullman, WA, 99164-7010 USA
Copyright 1995-2003 Washington State University